Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.107 extracted from

  • Sebert, M.E.; Patel, K.P.; Plotnick, M.; Weiser, J.N.
    Pneumococcal HtrA protease mediates inhibition of competence by the CiaRH two-component signaling system (2005), J. Bacteriol., 187, 3969-3979.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.107

Activating Compound

Activating Compound Comment Organism Structure
IPTG
-
 Streptococcus pneumoniae

Application

Application Comment Organism
additional information regulatory role for pneumococcal HtrA in modulating the activity of a two-component signaling system that controls the development of genetic competence Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as an IPTG-inducible, recombinant protein having both N-terminal and C-terminal His6-tags Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information mutant P1149 containing an insertion deletion mutation in the caiRH locus resulting in substantially lower HtrA expression Streptococcus pneumoniae
S234A mutant P1386, release of competence inhibition when mutant P1388 is present alone Streptococcus pneumoniae
T230P mutant P1388 with ciaH mutation Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
additional information rHtrA-mediated decline in transformation efficiency can not be corrected with excess competence-stimulating peptide Streptococcus pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Streptococcus pneumoniae 9986
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE with coomassie staining Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni affinity chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-casein + H2O
-
 Streptococcus pneumoniae ?
-
 ?

Synonyms

Synonyms Comment Organism
HtrA protease
-
 Streptococcus pneumoniae
HtrA/DegP
-
 Escherichia coli
serine protease
-
 Streptococcus pneumoniae