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Literature summary for 3.4.21.106 extracted from

  • Raevskaya, A.A.; Kuznetsova, E.M.; Savvateeva, M.V.; Severin, S.E.
    Isolation, purification, and study of properties of recombinant hepsin from Escherichia coli (2010), Biochemistry, 75, 866-872.
    View publication on PubMed
Search for more literature for 3.4.21.106

Activating Compound

Activating Compound Comment Organism Structure
resveratrol hepsin activity increases in dose-dependent manner in the presence of resveratrol Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strains BL21(DE3), BL21(DE3) Codon Plus RIL, and Rosetta pLysS Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
anthralin at 0.067 mM anthralin the hepsin activity is reduced by more than 70% Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 L-Asp-L-Lys-(gamma-Cbo)-L-Pro-L-Arg-4-nitroanilide in 0.3M Tris-HCl, 0.3 M imidazole, and 0.5 M NaCl, pH 8.4, at 37°C Homo sapiens
0.05
-
 L-Asp-L-Ala-L-Ala-L-Arg-4-nitroanilide in 0.3M Tris-HCl, 0.3 M imidazole, and 0.5 M NaCl, pH 8.4, at 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42038
-
 x * 42038, calculated from amino acid sequence Homo sapiens
43000
-
 x * 43000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-IDA.Sepharose column chromatography and Q-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Asp-L-Ala-L-Ala-L-Arg-4-nitroanilide + H2O
-
 Homo sapiens L-Asp-L-Ala-L-Ala-L-Arg + 4-nitroaniline
-
 ?
L-Asp-L-Lys-(gamma-Cbo)-L-Pro-L-Arg-4-nitroanilide + H2O
-
 Homo sapiens L-Asp-L-Lys-(gamma-Cbo)-L-Pro-L-Arg + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Homo sapiens
? x * 42038, calculated from amino acid sequence Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens calculated from amino acid sequence
-
 7.3