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Literature summary for 3.4.21.106 extracted from

  • Beliveau, F.; Desilets, A.; Leduc, R.
    Probing the substrate specificities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched fluorescent peptides (2009), FEBS J., 276, 2213-2226.
    View publication on PubMed
Search for more literature for 3.4.21.106

Cloned(Commentary)

Cloned (Comment) Organism
fragment containing amino acids 45-417 is expressed as a C-terminal His-tagged fusion protein in Drosophila S2 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)-benzenesulfonylfluoride hydrochloride residual hepsin activity: 0% Homo sapiens
4-methylumbelliferyl p-guanidinobenzoate irreversible inhibitor Homo sapiens
alpha1-Aantichymotrypsin residual hepsin activity: 88% Homo sapiens
alpha1-antitrypsin residual hepsin activity: 67% Homo sapiens
alpha2-antiplasmin residual hepsin activity: 1% Homo sapiens
antithrombin III residual hepsin activity: 0% Homo sapiens
Aprotinin residual hepsin activity: 1% Homo sapiens
leupeptin residual hepsin activity: 4% Homo sapiens
plasminogen activator inhibitor-1 residual hepsin activity: 0% Homo sapiens
Soybean trypsin inhibitor residual hepsin activity: 78% Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
 Abz-RQLRVVGGY(3-NO2)
-
 Homo sapiens
0.07
-
 Abz-RQRRVVGGY(3-NO2)
-
 Homo sapiens
0.072
-
 Abz-RRARVVGGY(3-NO2)
-
 Homo sapiens
0.085
-
 Abz-RQRRALEKY(3-NO2) peptide sequence from alphaEbeta7 integrin Homo sapiens
0.109
-
 Abz-RQYRVVGGY(3-NO2)
-
 Homo sapiens
0.114
-
 Abz-KQSRKFVPY(3-NO2) peptide sequence from trask Homo sapiens
0.14
-
 Abz-SKLRVVGGY(3-NO2) peptide sequence from proMSP-1 Homo sapiens
0.175
-
 Abz-RQARYVGGY(3-NO2)
-
 Homo sapiens
0.189
-
 Abz-RKRRGSRGY(3-NO2) peptide sequence from filaggrin Homo sapiens
0.191
-
 Abz-RQARAVGGY(3-NO2)
-
 Homo sapiens
0.22
-
 Abz-RAARVVGGY(3-NO2)
-
 Homo sapiens
0.29
-
 Abz-RLARVVGGY(3-NO2)
-
 Homo sapiens
0.369
-
 Abz-RQARVVGGY(3-NO2)
-
 Homo sapiens
0.369
-
 Abz-RQARVVGGY(3-NO2) peptide sequence from matriptase Homo sapiens
0.373
-
 Abz-SKGRSLIGY(3-NO2) peptide sequence from PAR-2 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 SDS-PAGE, autocatalytically processed form consisting of amino acids 163-417 Homo sapiens
45000
-
 SDS-PAGE, molecular weight of used fragment containing amino acids 45-417 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using HisTrap FF columns Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver hepsin is cloned from a liver cDNA Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-KQSRKFVPY(3-NO2) + H2O peptide sequence from trask Homo sapiens Abz-KQSR + KFVPY(3-NO2)
-
 ?
Abz-RAARVVGGY(3-NO2) + H2O
-
 Homo sapiens Abz-RAAR + VVGGY(3-NO2)
-
 ?
Abz-RKRRGSRGY(3-NO2) + H2O peptide sequence from filaggrin Homo sapiens Abz-RKRR + GSRGY(3-NO2)
-
 ?
Abz-RLARVVGGY(3-NO2) + H2O
-
 Homo sapiens Abz-RLAR + VVGGY(3-NO2)
-
 ?
Abz-RQARAVGGY(3-NO2) + H2O
-
 Homo sapiens Abz-RQAR + AVGGY(3-NO2)
-
 ?
Abz-RQARVVGGY(3-NO2) + H2O
-
 Homo sapiens Abz-RQAR + VVGGY(3-NO2)
-
 ?
Abz-RQARVVGGY(3-NO2) + H2O peptide sequence from matriptase Homo sapiens Abz-RQAR + VVGGY(3-NO2)
-
 ?
Abz-RQARYVGGY(3-NO2) + H2O
-
 Homo sapiens Abz-RQAR + YVGGY(3-NO2)
-
 ?
Abz-RQLRVVGGY(3-NO2) + H2O
-
 Homo sapiens Abz-RQLR + VVGGY(3-NO2)
-
 ?
Abz-RQRRALEKY(3-NO2) + H2O peptide sequence from alphaEbeta7 integrin Homo sapiens Abz-RQRR + ALEKY(3-NO2)
-
 ?
Abz-RQRRVVGGY(3-NO2) + H2O
-
 Homo sapiens Abz-RQRR + VVGGY(3-NO2)
-
 ?
Abz-RQYRVVGGY(3-NO2) + H2O
-
 Homo sapiens Abz-RQYR + VVGGY(3-NO2)
-
 ?
Abz-RRARVVGGY(3-NO2) + H2O
-
 Homo sapiens Abz-RRAR + VVGGY(3-NO2)
-
 ?
Abz-SKGRSLIGY(3-NO2) + H2O peptide sequence from PAR-2 Homo sapiens Abz-SKGR + SLIGY(3-NO2)
-
 ?
Abz-SKLRVVGGY(3-NO2) + H2O peptide sequence from proMSP-1 Homo sapiens Abz-SKLR + VVGGY(3-NO2)
-
 ?
Boc-Gln-Ala-Arg-AMC + H2O
-
 Homo sapiens ?
-
 ?
additional information to study the substrate specificity of type II transmembrane serine proteases internally quenched fluorogenic peptide substrates are used based on the autoactivation sequence of matriptase (RQARVVGG). Positions P4, P3, P2 and P1’ are substituted with nonpolar (Ala, Leu), aromatic (Tyr), acid (Glu) and basic (Arg) amino acids, whereas P1 is fixed to Arg. Hepsin shares similarities with matriptase and DESC1, but is markedly more permissive at P2 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
hepsin
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 Abz-RAARVVGGY(3-NO2)
-
 Homo sapiens
0.5
-
 Abz-RRARVVGGY(3-NO2)
-
 Homo sapiens
0.5
-
 Abz-RQARAVGGY(3-NO2)
-
 Homo sapiens
0.5
-
 Abz-RQARYVGGY(3-NO2)
-
 Homo sapiens
0.6
-
 Abz-RLARVVGGY(3-NO2)
-
 Homo sapiens
1.1
-
 Abz-RQYRVVGGY(3-NO2)
-
 Homo sapiens
1.4
-
 Abz-RQARVVGGY(3-NO2)
-
 Homo sapiens
1.4
-
 Abz-RQRRVVGGY(3-NO2)
-
 Homo sapiens
1.4
-
 Abz-RQARVVGGY(3-NO2) peptide sequence from matriptase Homo sapiens
1.5
-
 Abz-RQLRVVGGY(3-NO2)
-
 Homo sapiens
12
-
 Abz-KQSRKFVPY(3-NO2) peptide sequence from trask Homo sapiens
15
-
 Abz-SKGRSLIGY(3-NO2) peptide sequence from PAR-2 Homo sapiens
16
-
 Abz-SKLRVVGGY(3-NO2) peptide sequence from proMSP-1 Homo sapiens
39
-
 Abz-RQRRALEKY(3-NO2) peptide sequence from alphaEbeta7 integrin Homo sapiens
66
-
 Abz-RKRRGSRGY(3-NO2) peptide sequence from filaggrin Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Homo sapiens