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Literature summary for 3.4.21.106 extracted from
- Hepsin and prostate cancer (2007), Front. Biosci., 12, 5052-5059.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | hepsin is not necessary for embryonic development of mice and postnatal survival and is dispensable for normal hemostasis and liver function | Mus musculus |
| medicine | hepsin is one of the most upregulated genes in prostate cancer. Upregulation appears to correlate with disease progression. Role of hepsin is more important in cancer cell migration/invasion than cell proliferation. Hepsin mRNA expression appears to be lower in hormone-refractory prostate cancer compared to clinically localized prostate cancer. Single nucleotide polymorphisms are associated with prostate cancer susceptibility. In contrast, prostate cancer cell lines stably transfected with a hepsin expressing plasmid grow slower and are less invasive in culture. Similarly, stable transfection of hepsin cDNA in ovarian cancer cell lines promotes apoptosis and inhibits tumor cell growth | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Mus musculus |
| prostate cancer cell lines stably transfected with a hepsin expressing plasmid. Stable transfection of hepsin cDNA in ovarian cancer cell lines. Overexpression in transgenic mice | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| has scavenger receptor-like cysteine-rich domain and a large loop between residues 241 and 256, which may represent a major determinant for its substrate recognition | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | hepsin-deficient mice are viable and fertile, grow normally, have no detectable defects in liver histology and biochemistry. Blood clotting and tail bleeding times are also normal. Serum concentrations of bone-derived alkaline phosphatase are ca. 2fold higher in hepsin-deficient mice than in that of control mice | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | hepatocyte growth factor activator inhibitor 1 and hepatocyte growth factor activator inhibitor 2 | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | has an integral transmembrane domain near the N-terminus | Homo sapiens | 16020 | - |
| soluble | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
x * 51000, SDS-PAGE and Western blot analysis | Homo sapiens |
| 51000 | - |
x * 51000, SDS-PAGE and Western blot analysis | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| breast cancer cell | upregulation of hepsin mRNA in estrogen receptor alpha-positive breast tumors compared with estrogen receptor alpha-negative breast tumors | Homo sapiens | - |
| Hep-G2 cell | - |
Homo sapiens | - |
| hepatocyte | abundant | Homo sapiens | - |
| kidney | lower levels | Homo sapiens | - |
| liver | - |
Mus musculus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| ovary cancer cell line | elevated hepsin mRNA expression levels in ca. 58% of low grade tumors and ca. 84% of more advanced ovarian carcinomas. Hepsin mRNA expression is significantly higher in primary ovarian serous papillary carcinomas compared to that in omental metastasis | Homo sapiens | - |
| pancreas | lower levels | Homo sapiens | - |
| prostate | lower levels | Homo sapiens | - |
| prostate cancer cell | upregulation of hepsin | Homo sapiens | - |
| renal cell carcinoma cell | hepsin mRNA levels in early stage cancer are similar to that in normal controls but mRNA levels decrease in more advanced cancers. Patients with lower hepsin mRNA levels have poorer survival rate. In contrast, another study reports lower hepsin mRNA expression in early stage cancers, in some cases of advanced cancer overexpression of hepsin mRNA and patients with high hepsin mRNA levels with poorer survival rates | Homo sapiens | - |
| thyroid gland | lower levels | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| blood clotting factor IX + H2O | - |
Homo sapiens | active blood clotting factor IX | - |
? |  |
| blood clotting factor VII + H2O | - |
Homo sapiens | active blood clotting factor VII | - |
? | |
| blood clotting factor XII + H2O | - |
Homo sapiens | active blood clotting factor XII | - |
? | |
| additional information | hepsin exhibits trypsin-like catalytic activity that favores Arg at the P1, Thr/Leu/Asn at the P2, Gln/Lys at the P3, and Pro/Lys at the P4 positions | Homo sapiens | ? | - |
? | |
| pro-hepatocyte growth factor + H2O | hepsin-mediated pro-HGF activation may be critical in the pathogenesis of human prostate cancer | Homo sapiens | active hepatocyte growth factor | - |
? | |
| pro-urokinase + H2O | - |
Homo sapiens | active urokinase | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51000, SDS-PAGE and Western blot analysis | Homo sapiens |
| ? | x * 51000, SDS-PAGE and Western blot analysis | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| type II transmembrane serine protease | - |
Mus musculus |
| type II transmembrane serine protease | - |
Homo sapiens |
| type II transmembrane serine protease | - |
Rattus norvegicus |
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