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Literature summary for 3.4.21.106 extracted from

  • Li, Y.; Yu, Z.; Zhao, X.; Shen, S.
    Identification and characterization of hepsin/-TM, a non-transmembrane hepsin isoform (2005), Biochim. Biophys. Acta, 1681, 157-165.
    View publication on PubMed
Search for more literature for 3.4.21.106

Application

Application Comment Organism
medicine expression of the non-transmembrane isoform in vivo does not exert any apparent inhibitory effect on cell growth Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
transmembrane isoform and non-transmembrane isoform cloned into vector pcDNA3/Myc-His-(-C), both isoforms expressed in BHK-21 cells, non-transmembrane isoform expressed in bacteria strain DE3, overexpression of myc-tagged non-transmembrane hepsin in HEK-293 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm non-transmembrane isoform Homo sapiens 5737
-
membrane transmembrane isoform Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 amino sequence analysis in both non-reduction and reduction conditions of non-transmembrane isoform Homo sapiens
45000
-
 Western blot analysis, SDS-PAGE, transmembrane isoform expressed in HEK-293T cells, amino sequence analysis of transmembrane isoform Homo sapiens
75000
-
 Western blot analysis, SDS-PAGE, non-transmembrane isoform expressed in HEK-293T cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain non-transmembrane isoform highly expressed, whereas transmembrane hepsin is expressed at a relatively lower level Homo sapiens
-
Caco-2 cell non-transmembrane isoform expressed at highest level among investigated cell lines, transmembrane isoform hardly detectable Homo sapiens
-
colonic adenocarcinoma cell line
-
 Homo sapiens
-
heart transmembrane isoform only Homo sapiens
-
HEK-293T cell two isoforms expressed with different molecular weight Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
kidney non-transmembrane and transmembrane isoform Homo sapiens
-
liver transmembrane isoform only Homo sapiens
-
LS174T cell non-transmembrane and transmembrane isoform highly expressed Homo sapiens
-
lung non-transmembrane and transmembrane isoform Homo sapiens
-
additional information both isoforms hardly detectable in SW-1417 cells, Colo-201 cells, Colo-205 cells, SW-1222 cells, LS-180 cells, HT-29 cell and LoVo cells Homo sapiens
-
skeletal muscle transmembrane isoform only Homo sapiens
-
spleen transmembrane isoform only Homo sapiens
-
SW-403 cell non-transmembrane and transmembrane isoform highly expressed Homo sapiens
-
thymus transmembrane isoform only Homo sapiens
-