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Literature summary for 3.4.21.106 extracted from

  • Zhukov, A.; Hellman, U.; Ingelman-Sundberg, M.
    Purification and characterization of hepsin from rat liver microsomes (1997), Biochim. Biophys. Acta, 1337, 85-95.
    View publication on PubMed
Search for more literature for 3.4.21.106

Activating Compound

Activating Compound Comment Organism Structure
pepstatin A 21% activation at 0.1 mM Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin 94% inhibition at 0.1 mM Rattus norvegicus
diisopropylfluorophosphate complete inhibition at 5 mM Rattus norvegicus
leupeptin 99% inhibition at 0.1 mM Rattus norvegicus
phenylmethanesulfonyl fluoride 80% inhibition at 1 mM Rattus norvegicus
ZnSO4 26% inhibition at 1 mM Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rattus norvegicus 16020
-
microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 1 * 31000 + 1 * 19000, SDS-PAGE under reducing conditions Rattus norvegicus
31000
-
 1 * 31000 + 1 * 19000, SDS-PAGE under reducing conditions Rattus norvegicus
50000
-
 zymogen Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Protein + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification zymogen is converted to the active protease by cleavage between Arg161 and Ile162 Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
homogeneity Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.67
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cleaves after basic amino acid residues, Arg being preferable to Lys Rattus norvegicus ?
-
 ?
N-benzyloxycarbonyl-Ala-Arg-Arg 4-methylcoumarin 7-amide + H2O weak substrate Rattus norvegicus N-benzyloxycarbonyl-Ala-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
N-tert-butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide + H2O weak substrate Rattus norvegicus N-tert-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
Protein + H2O
-
 Rattus norvegicus ?
-
 ?
tert-butyloxycarbonyl-Gln-Ala-Arg 4-methylcoumarin 7-amide + H2O weak substrate Rattus norvegicus tert-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus tert-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O weak substrate Rattus norvegicus tert-Butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 31000 + 1 * 19000, SDS-PAGE under reducing conditions Rattus norvegicus

Synonyms

Synonyms Comment Organism
S01.224 Merops ID Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 activity with in the range pH 6-9 has no maximum, the activity increases continuously Rattus norvegicus