Crystallization (Comment) | Organism |
---|---|
structure of Escherichia coli GlpG consists of a 6-transmembrane domain topology | Toxoplasma gondii |
structure of Escherichia coli GlpG consists of a 6-transmembrane domain topology | Escherichia coli |
structure of Escherichia coli GlpG consists of a 6-transmembrane domain topology. The active site of Escherichia coli GlpG is found at the bottom of a shallow pocket that faces the extracellular side of the membrane. Above the catalytic dyad is a loop structure which lies roughly parallel to the membrane plane. A side chain from the loop (Phe-245) drops down and seals a gap between two transmembrane helices. This loop structure sterically hinders substrate access to the catalytic serine and is intrinsically flexible | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
H2541X | using mutagenesis it is shown that His254 is catalytically essential | Plasmodium falciparum |
H2541X | using mutagenesis it is shown that His254 is catalytically essential | Toxoplasma gondii |
H2541X | using mutagenesis it is shown that His254 is catalytically essential | Escherichia coli |
S201X | using mutagenesis it is shown that Ser201 is catalytically essential | Plasmodium falciparum |
S201X | using mutagenesis it is shown that Ser201 is catalytically essential | Toxoplasma gondii |
S201X | using mutagenesis it is shown that Ser201 is catalytically essential | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Drosophila melanogaster | 16020 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
growth-factor gurken + H2O | Drosophila melanogaster | - |
? | - |
? | |
growth-factor spitz + H2O | Drosophila melanogaster | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Escherichia coli | P09391 | - |
- |
Plasmodium falciparum | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Toxoplasma gondii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adhesin EBP-175 + H2O | adhesin EBP-175 of Plasmodium falciparum undergoes ectodomain shedding, in a reaction catalyzed by plasmodium rhomboid pfROM4. pfROM4 cleaves within the transmembrane region of the adhesin | Plasmodium falciparum | ? | - |
? | |
adhesin MIC2 + H2O | the ectodomain of Toxoplasma gondii adhesin MIC2, a type-I membrane protein is cleaved by rhomboid | Toxoplasma gondii | ? | - |
? | |
Ccp1 + H2O | Ccp1 is a mitochondrial cytochrome c peroxidase its cleavage side resides in a short stretch of moderately hydrophobic sequence | Saccharomyces cerevisiae | ? | - |
? | |
growth-factor gurken + H2O | - |
Drosophila melanogaster | ? | - |
? | |
growth-factor spitz + H2O | - |
Drosophila melanogaster | ? | - |
? | |
Mgm1 + H2O | Mgm1 is a dynamin-like GTPase its cleavage side resides in a short stretch of moderately hydrophobic sequence | Saccharomyces cerevisiae | ? | - |
? | |
additional information | an artificial fusion protein bearing the sequence around the second transmembrane domain of LacY is cleavable by Escherichia coli GlpG in intact bacterial cells (LacY itself is not a substrate for rhomboid). A Ser-Asp bond is cleaved | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlpG | - |
Escherichia coli |
pfROM4 | - |
Plasmodium falciparum |
Rhomboid | - |
Drosophila melanogaster |
rhomboid Pcp1/Rbd1 | - |
Saccharomyces cerevisiae |
TgROM5 | - |
Toxoplasma gondii |
General Information | Comment | Organism |
---|---|---|
malfunction | rhomboid is required for pattern formation in the ventral ectoderm, in which mutation causes a rhomboid-shaped head skeleton. This phenotype is due to the role of fly rhomboid in epidermal growth factor receptor signaling | Drosophila melanogaster |