Any feedback?
Literature summary for 3.4.21.105 extracted from
- An Entamoeba histolytica rhomboid protease with atypical specificity cleaves a surface lectin involved in phagocytosis and immune evasion (2008), Genes Dev., 22, 1636-1646.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transiently expressed in COS, HeLa, HEK 293, and CHO cells as a N-terminal hemagglutinin tagged fusion protein | Entamoeba histolytica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | activity is abolished with a catalytic serine to alanine mutant | Entamoeba histolytica |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| integral to membrane | EhROM1 is an active intramembrane serine protease | Entamoeba histolytica | - |
- |
| additional information | EhROM1 changes localization to vesicles during phagocytosis and to the posterior cap structure during surface receptor shedding for immune evasion, in both cases colocalizing with lectins | Entamoeba histolytica | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
Western blot | Entamoeba histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lectin + H2O | Entamoeba histolytica | EhROM1 is able to cleave cell surface lectin | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adhesin + H2O | EhROM1 is able to cleave Plasmodium adhesins but not the canonical substrate Drosophila Spitz. It is examined whether EhROM1 can cleave a representative of each of the four families of Plasmodium adhesins: the EBL adhesin BAEBL, the RBL adhesin Rh4, AMA1, and TRAP. All adhesins are efficient substrates for the recoded EhROM1 with the exception of AMA1, which is cleaved less well than the others by EhROM1 | Entamoeba histolytica | ? | - |
? | |
| lectin + H2O | EhROM1 is able to cleave cell surface lectin | Entamoeba histolytica | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EhROM1 | - |
Entamoeba histolytica |
| rhomboid protease | - |
Entamoeba histolytica |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
