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Literature summary for 3.4.21.105 extracted from
- Reconstitution of intramembrane proteolysis in vitro reveals that pure rhomboid is sufficient for catalysis and specificity (2005), Proc. Natl. Acad. Sci. USA, 102, 1883-1888.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dichloroisocoumarin | below 0.1 mM | Aquifex aeolicus |  |
| dichloroisocoumarin | below 0.1 mM | Bacillus subtilis | |
| dichloroisocoumarin | below 0.1 mM | Escherichia coli | |
| dichloroisocoumarin | below 0.1 mM | Providencia stuartii | |
| additional information | no inhibition by EDTA, o-phenanthroline, E64, PMSF, 4-(2-aminoethyl)benzenesulfonyl fluoride and pepstatin A | Aquifex aeolicus | |
| additional information | no inhibition by EDTA, o-phenanthroline, E64, PMSF, 4-(2-aminoethyl)benzenesulfonyl fluoride and pepstatin A | Bacillus subtilis | |
| additional information | no inhibition by EDTA, o-phenanthroline, E64, PMSF, 4-(2-aminoethyl)benzenesulfonyl fluoride and pepstatin A | Escherichia coli | |
| additional information | no inhibition by EDTA, o-phenanthroline, E64, PMSF, 4-(2-aminoethyl)benzenesulfonyl fluoride and pepstatin A | Providencia stuartii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Providencia stuartii | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| C100Spi-Flag + H2O | no cleavage of C100-Flag | Bacillus subtilis | ? | - |
? | |
| C100Spi-Flag + H2O | no cleavage of C100-Flag | Escherichia coli | ? | - |
? | |
| C100Spi-Flag + H2O | no cleavage of C100-Flag | Providencia stuartii | ? | - |
? | |
| C100Spi-Flag + H2O | no cleavage of C100-Flag | Aquifex aeolicus | ? | - |
? | |
| chimeric protein of the bacterial pelB leader peptide, GFP as the extracellularectodomain, the juxtamembrane-transmembrane-cytosolic residues 122-230 of Spitz and a C-terminal epitope + H2O | - |
Bacillus subtilis | ? | - |
? | |
| chimeric protein of the bacterial pelB leader peptide, GFP as the extracellularectodomain, the juxtamembrane-transmembrane-cytosolic residues 122-230 of Spitz and a C-terminal epitope + H2O | - |
Providencia stuartii | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AarA | - |
Providencia stuartii |
| AqRho | - |
Aquifex aeolicus |
| GlpG | - |
Escherichia coli |
| Yqgp | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 37 | more active at 25°C than at 37°C | Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 37 | much more active az 37°C than at 25°C | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme does not require cofactors to catalyze intramembrane proteolysis | Bacillus subtilis | |
| additional information | enzyme does not require cofactors to catalyze intramembrane proteolysis | Escherichia coli | |
| additional information | enzyme does not require cofactors to catalyze intramembrane proteolysis | Providencia stuartii | |
| additional information | enzyme does not require cofactors to catalyze intramembrane proteolysis | Aquifex aeolicus |
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