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Literature summary for 3.4.21.104 extracted from

  • Thiel, S.; Kolev, M.; Degn, S.; Steffensen, R.; Hansen, A.G.; Ruseva, M.; Jensenius, J.C.
    Polymorphisms in mannan-binding lectin (MBL)-associated serine protease 2 affect stability, binding to MBL, and enzymatic activity (2009), J. Immunol., 182, 2939-2947.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
H-ficolin
-
Homo sapiens
L-ficolin
-
Homo sapiens
M-ficolin
-
Homo sapiens
mannan-binding lectin
-
Homo sapiens
additional information MASP-2 is activated by the cleavage of the 76-kDa polypeptide chain into a 52-kDa A chain and a 31-kDa B chain Homo sapiens

Protein Variants

Protein Variants Comment Organism
D120G when D120G is mixed with mannan-binding lectin, no activation of complement component C4 is observed Homo sapiens
H155R the mutant cleaves complement component C4 slightly better than the wild type MASP-2 Homo sapiens
additional information when the mutant enzyme 156_159dupCHNH (CHNHdup) is mixed with mannan-binding lectin, no activation of complement component C4 is observed Homo sapiens
P126L the mutant cleaves complement component C4 with an activity comparable to wild type MASP-2 Homo sapiens
R439H the mutant is deficient in cleavage of complement component C4 despite its normal binding to mannan-binding lectin, the mutant is not able to autoactivate in the presence of mannan-binding lectin and mannan Homo sapiens
R99Q the mutant cleaves complement component C4 with an activity comparable to wild type MASP-2 Homo sapiens
V377A the mutant cleaves complement component C4 with an activity comparable to wild type MASP-2 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
Homo sapiens
-
HEK-293 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
complement component C4 + H2O
-
Homo sapiens complement component C4b + ?
-
?

Synonyms

Synonyms Comment Organism
MASP-2
-
Homo sapiens
MBL-associated serine protease 2
-
Homo sapiens