Literature summary for 3.4.21.104 extracted from

Girija, U.V.; Dodds, A.W.; Roscher, S.; Reid, K.B.; Wallis, R.
Localization and characterization of the mannose-binding lectin (MBL)-associated-serine protease-2 binding site in rat ficolin-A: equivalent binding sites within the collagenous domains of MBLs and ficolins (2007), J. Immunol., 179, 455-462.

Search for more literature for 3.4.21.104

Activating Compound

Activating Compound	Comment	Organism	Structure
ficolin	MASP-2 interactions and activation activity with wild-type and mutant ficolins, overview	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
expression of catalytically active and inactive wild-type and mutant MASP-2 zymogens in CHO cells, enzyme secretion	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
R424K	site-directed mutagenesis, the mutant shows a reduced autoactivation rate, due to more slowly autoactivation reaction compared to the wild-type enzyme, with reduced zymogen activation during biosynthesis, secretion, and purification	Rattus norvegicus
S613A	site-directed mutagenesis, the mutant zymogen cannot autoactivate and is secreted in the zymogen form	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics of MASP-2 interactions with wild-type and mutant ficolins, overview	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
secreted recombinant catalytically active and inactive wild-type and mutant MASP-2 zymogens from CHO cell culture medium	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	MASP-2 interactions with wild-type and mutant ficolins, overview	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
mannose-binding lectin-associated-serine protease-2	-	Rattus norvegicus
MASP-2	-	Rattus norvegicus
MBL-associated-serine protease-2	-	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Rattus norvegicus

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Release 2023.1 (January 2023)