Literature summary for 3.4.21.104 extracted from

Gal, P.; Harmat, V.; Kocsis, A.; Bian, T.; Barna, L.; Ambrus, G.; Vegh, B.; Balczer, J.; Sim, R.B.; Naray-Szabo, G.; Zavodszky, P.
A true autoactivating enzyme. Structural insight into mannose-binding lectin-associated serine protease-2 activations (2005), J. Biol. Chem., 280, 33435-33444.

Search for more literature for 3.4.21.104

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
zymogen and the activated form, 2.4 A resolution	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
MASP-2 CCP1-CCP2-SP R444Q	lower KM-value for complement C4	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
complement C1 inhibitor	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000377	-	Complement component C4	mutant MASP-2 CCP1-CCP2-SP R444Q	Homo sapiens
0.0016	-	Complement component C4	MASP-2 CCP1-CCP2-SP	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	x * 44000, mutant MASP-2 CCP1-CCP2-SP R444Q, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O00187	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzyloxycarbonyl-Gly-Arg-S-benzyl + H2O	-	Homo sapiens	?	-	?
complement component C4 + H2O	-	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 44000, mutant MASP-2 CCP1-CCP2-SP R444Q, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
mannan-binding lectin-associated serine protease-2	-	Homo sapiens
MASP-2	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
48.2	-	Tm for the zymogen of mutant MASP-2 CCP1-CCP2-SP R444Q	Homo sapiens
50.8	-	Tm for the active fragment of mutant MASP-2 CCP1-CCP2-SP R444Q	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.026	-	Complement component C4	mutant MASP-2 CCP1-CCP2-SP R444Q	Homo sapiens
0.9	-	Complement component C4	MASP-2 CCP1-CCP2-SP	Homo sapiens

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	-	wild-type zymogen MASP-2	Homo sapiens

pI Value

Organism	Comment	pI Value Maximum	pI Value
Homo sapiens	-	-	5.6

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Release 2023.1 (January 2023)