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Literature summary for 3.4.21.104 extracted from
- Production and purification of recombinants of mouse MASP-2 and sMAP (2005), J. Endotoxin Res., 11, 47-50.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression using a Drosophila melanogaster expression system, C4-cleavage activity reconstitution of the enzyme-deficient mutant by addition of recombinant enzyme to deficient serum | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of MASP-2-deficient mice | Mus musculus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Mus musculus |  |
| Na+ | - |
Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
x * 87000, proenzyme form, SDS-PAGE, 1 * 52000, heavy enzyme chain, active enzyme form, SDS-PAGE | Mus musculus |
| 87000 | - |
x * 87000, proenzyme form, SDS-PAGE, 1 * 52000, heavy enzyme chain, active enzyme form, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| complement component C4 + H2O | Mus musculus | - |
2 fragments of complement C4 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | autoactivation, autoactivation of the recombinant enzyme during purification | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| complement component C4 + H2O | - |
Mus musculus | 2 fragments of complement C4 | - |
? | |
| complement component C4 + H2O | activated MBL-MASP-2 complex | Mus musculus | 2 fragments of complement C4 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 87000, proenzyme form, SDS-PAGE, 1 * 52000, heavy enzyme chain, active enzyme form, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MASP-2 | - |
Mus musculus |
| MBL-associated serine protease | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
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Release 2023.1 (January 2023)
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