Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Diazoacetyl-DL-norleucine methyl ester | 0.042 mg/ml, 95% inhibition | Physarum polycephalum | |
PMSF | 10 mM, 21% inhibition | Physarum polycephalum | |
tosyl-L-Leu-chloromethylketone | 10 mM, 18% inhibition | Physarum polycephalum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Physarum polycephalum | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 31000 + 1 * 23000, the heavy and the light chain are cross-linked by disulfide bonds, SDS-PAGE | Physarum polycephalum |
31000 | - |
1 * 31000 + 1 * 23000, the heavy and the light chain are cross-linked by disulfide bonds, SDS-PAGE | Physarum polycephalum |
54000 | - |
- |
Physarum polycephalum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physarum polycephalum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the heavy chain, but not the light chain is glycosylated. One molecule of native enzyme contains about 7 residues of mannose, 5 residues of glucosamine, and 1 residue each of fucose and glucose | Physarum polycephalum |
Purification (Comment) | Organism |
---|---|
- |
Physarum polycephalum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasmodium | - |
Physarum polycephalum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hemoglobin + H2O | - |
Physarum polycephalum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 31000 + 1 * 23000, the heavy and the light chain are cross-linked by disulfide bonds, SDS-PAGE | Physarum polycephalum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
pH 3.0-6.0, stable | Physarum polycephalum |
37 | - |
pH 8.0, 15 min, 30% loss of activity | Physarum polycephalum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
1.7 | - |
hydrolysis of hemoglobin | Physarum polycephalum |