BRENDA - Reference to 3.4.21.102; Id = 731635

additional information

a peptide ligand, which copurifies and cocrystallizes with CtpB, is observed at the entrance of the protease tunnel near the catalytic triad, its binding to the PDZ domain stimulates the protease activity

Bacillus subtilis

expression of the His-tagged enzyme in Escherichia coli strains BL21(DE3)pLysS or B834(DE3)

Bacillus subtilis

purified recombinant enzyme, all CtpB crystals are grown at 19°C using sitting-drop vapor-diffusion method, X-ray diffraction structure determination and crystal structure analysis

Bacillus subtilis

membrane protein SpoIVFA + H2O

Bacillus subtilis

sequenctial processing by the enzyme together with protease 4B. The 4FA regulatory protein is composed of a globular N-terminal domain, a transmembrane helix (residues 73-90), an unstructured linker region and a compact C-terminal domain (residues 160-255, LytM-like) extending into the intermembrane space. 4B/CtpB-mediated removal of 4FA residues 131-154 induces sK maturation

?

-

?

Bacillus subtilis

O35002

-

recombinant His-tagged enzyme from Escherichia coli strains BL21(DE3)pLysS or B834(DE3) by nickel affinity chromatography and gel filtration

Bacillus subtilis

membrane protein SpoIVFA + H2O

-

731635

Bacillus subtilis

?

-

?

membrane protein SpoIVFA + H2O

sequenctial processing by the enzyme together with protease 4B. The 4FA regulatory protein is composed of a globular N-terminal domain, a transmembrane helix (residues 73-90), an unstructured linker region and a compact C-terminal domain (residues 160-255, LytM-like) extending into the intermembrane space. 4B/CtpB-mediated removal of 4FA residues 131-154 induces sK maturation

731635

Bacillus subtilis

?

-

?

dimer

the CtpB protease is organized as a dimeric ring with the catalytic site burried within a tunnel, domain organization of CtpB, overview

Bacillus subtilis

25

-

assay at

Bacillus subtilis

7.5

-

assay at

Bacillus subtilis

additional information

a peptide ligand, which copurifies and cocrystallizes with CtpB, is observed at the entrance of the protease tunnel near the catalytic triad, its binding to the PDZ domain stimulates the protease activity

Bacillus subtilis

expression of the His-tagged enzyme in Escherichia coli strains BL21(DE3)pLysS or B834(DE3)

Bacillus subtilis

purified recombinant enzyme, all CtpB crystals are grown at 19°C using sitting-drop vapor-diffusion method, X-ray diffraction structure determination and crystal structure analysis

Bacillus subtilis

membrane protein SpoIVFA + H2O

Bacillus subtilis

sequenctial processing by the enzyme together with protease 4B. The 4FA regulatory protein is composed of a globular N-terminal domain, a transmembrane helix (residues 73-90), an unstructured linker region and a compact C-terminal domain (residues 160-255, LytM-like) extending into the intermembrane space. 4B/CtpB-mediated removal of 4FA residues 131-154 induces sK maturation

?

-

?

recombinant His-tagged enzyme from Escherichia coli strains BL21(DE3)pLysS or B834(DE3) by nickel affinity chromatography and gel filtration

Bacillus subtilis

membrane protein SpoIVFA + H2O

-

731635

Bacillus subtilis

?

-

?

membrane protein SpoIVFA + H2O

sequenctial processing by the enzyme together with protease 4B. The 4FA regulatory protein is composed of a globular N-terminal domain, a transmembrane helix (residues 73-90), an unstructured linker region and a compact C-terminal domain (residues 160-255, LytM-like) extending into the intermembrane space. 4B/CtpB-mediated removal of 4FA residues 131-154 induces sK maturation

731635

Bacillus subtilis

?

-

?

dimer

the CtpB protease is organized as a dimeric ring with the catalytic site burried within a tunnel, domain organization of CtpB, overview

Bacillus subtilis

25

-

assay at

Bacillus subtilis

7.5

-

assay at

Bacillus subtilis

evolution

the enzyme CtpB belongs to the widespread family of PDZ-proteases

Bacillus subtilis

additional information

the CtpB protease is organized as a dimeric ring with the catalytic site burried within a tunnel. The PDZ domain controls access to the protease tunnel. Residues Ser309, Lys334, and Gln338 function as catalytic triad mediating cleavage of the 4FA substrate. A peptide ligand, which copurifies and cocrystallizes with CtpB, is observed at the entrance of the protease tunnel near the catalytic triad

Bacillus subtilis

physiological function

spore formation in Bacillus subtilis relies on a regulated intramembrane proteolysis pathway, involving the enzyme, that synchronizes mother-cell and forespore development. Activating protease CtpB assembles a gated protease tunnel regulating cell-cell signaling during spore formation. Activation of the SpoIV RIP pathway is induced by the concerted activity of enzyme CtpB and a second signaling protease, SpoIVB. Molecular basis of this SpoIV transmembrane signaling, the enzyme resembles a minimal version of a self-compartmentalizing protease regulated by a unique allosteric mechanism, overview

Bacillus subtilis

evolution

the enzyme CtpB belongs to the widespread family of PDZ-proteases

Bacillus subtilis

additional information

the CtpB protease is organized as a dimeric ring with the catalytic site burried within a tunnel. The PDZ domain controls access to the protease tunnel. Residues Ser309, Lys334, and Gln338 function as catalytic triad mediating cleavage of the 4FA substrate. A peptide ligand, which copurifies and cocrystallizes with CtpB, is observed at the entrance of the protease tunnel near the catalytic triad

Bacillus subtilis

physiological function

spore formation in Bacillus subtilis relies on a regulated intramembrane proteolysis pathway, involving the enzyme, that synchronizes mother-cell and forespore development. Activating protease CtpB assembles a gated protease tunnel regulating cell-cell signaling during spore formation. Activation of the SpoIV RIP pathway is induced by the concerted activity of enzyme CtpB and a second signaling protease, SpoIVB. Molecular basis of this SpoIV transmembrane signaling, the enzyme resembles a minimal version of a self-compartmentalizing protease regulated by a unique allosteric mechanism, overview

Bacillus subtilis

732180

Wegener

An atypical psbA gene encodes ...

Cyanothece sp. ATCC 51142

J. Biol. Chem.

290

3764-3774

2015

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1

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732467

Carroll

The lone S41 family C-terminal ...

Staphylococcus aureus

Microbiology

160

1737-1748

2014

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2

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732544

Deng

The periplasmic PDZ domain-con ...

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Mol. Plant Microbe Interact.

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101-112

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731635

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CtpB assembles a gated proteas ...

Bacillus subtilis

Cell

155

647-658

2013

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731957

Hernandez

Increased bile resistance in S ...

Salmonella enterica, Salmonella enterica SL1344

Int. Microbiol.

16

87-92

2013

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2

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1

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732822

Che

C-terminal processing of react ...

Arabidopsis thaliana

Proc. Natl. Acad. Sci. USA

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16247-16252

2013

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1

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1

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731328

Yokono

Alterations in photosynthetic ...

Synechocystis sp.

Biochim. Biophys. Acta

1817

754-759

2012

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732046

Sugiura

Environment of TyrZ in photosy ...

Thermosynechococcus elongatus

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The subcellular localization o ...

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316

23-30

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1

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2

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1

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5

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1

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717653

Zhang

Screening for inhibitors of pl ...

Spinacia oleracea

Hybridoma

30

223-227

2011

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1

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1

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1

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1

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1

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1

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1

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718206

Yue

A rice stromal processing pept ...

Oryza sativa Indica Group

Plant Cell Physiol.

51

475-485

2010

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1

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2

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1

4

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2

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3

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4

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1

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2

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1

4

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3

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4

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1

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718260

Zou

Expression of a serine proteas ...

Clonostachys rosea, Clonostachys rosea 611

PLoS ONE

5

e13386

2010

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1

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2

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1

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2

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1

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1

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2

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2

1

2

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710331

Booij-James

Nitric oxide donor-mediated in ...

Landoltia punctata

Planta

229

1347-1352

2009

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1

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4

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1

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1

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1

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679911

Gilbert

Mutagenesis of the carboxy ter ...

Rhizobium leguminosarum

FEMS Microbiol. Lett.

272

65-74

2007

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1

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1

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1

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1

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1

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1

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682255

Duff

-

The carboxyterminal processing ...

Spinacia oleracea

Pestic. Biochem. Physiol.

88

1-13

2007

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1

-

1

-

1

-

1

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1

-

3

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1

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1

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1

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1

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1

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3

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669103

Bandara

Carboxyl-terminal protease reg ...

Brucella suis bv. 1

J. Bacteriol.

187

5767-5775

2005

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1

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1

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2

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1

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9

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2

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1

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1

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1

-

2

-

1

-

2

-

1

-

670159

Reiling

Prc protease promotes mucoidy ...

Pseudomonas aeruginosa

Microbiology

151

2251-2261

2005

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1

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1

-

1

-

5

-

2

-

1

-

1

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1

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2

-

670483

Fabbri

The carboxyterminal processing ...

Spinacia oleracea

Pest Manag. Sci.

61

682-690

2005

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2

1

-

1

2

-

1

-

3

-

1

-

1

2

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5

1

-

1

-

2

1

-

1

2

-

1

-

1

2

-

5

1

-

1

-

670503

Richter

-

Function of the stromal proces ...

Anabaena sp., Arabidopsis thaliana, Chlamydomonas reinhardtii, Lotus japonicus, Medicago truncatula, Oryza sativa, Pisum sativum, Plasmodium falciparum, Synechocystis sp.

Physiol. Plant.

123

362-368

2005

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6

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2

-

9

-

11

-

9

-

9

-

20

1

-

6

-

2

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9

-

11

-

20

1

-

669139

Tadokoro

Interaction of the Escherichia ...

Escherichia coli

J. Biochem.

135

185-191

2004

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1

-

1

-

2

-

2

-

1

-

1

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2

-

650389

Kanervo

Dissecting a cyanobacterial pr ...

Synechocystis sp., Synechocystis sp. 6803

Biochim. Biophys. Acta

1607

131-140

2003

-

1

-

6

-

1

-

2

1

-

1

-

1

-

2

1

-

652464

Richter

Structural properties of the c ...

Pisum sativum

J. Biol. Chem.

278

39497-39502

2003

-

2

-

1

-

1

-

1

-

2

-

1

-

653494

Chaal

A thylakoidal processing pepti ...

Heterosigma akashiwo

Plant Mol. Biol.

52

463-472

2003

-

1

2

-

1

-

2

-

1

-

2

-

2

-

137246

Inagaki

Amino acid residues that are c ...

Synechocystis sp.

J. Biol. Chem.

276

30099-30105

2001

-

13

-

1

-

4

-

3

-

13

-

1

-

3

-

137247

Yamamoto

Overexpression and characteriz ...

Spinacia oleracea

J. Biol. Chem.

276

7518-7525

2001

-

1

-

1

-

1

-

3

-

1

-

3

1

-

1

-

1

-

1

-

1

-

1

-

1

-

3

1

-

1

-

137238

Beebe

Substrate recognition through ...

Escherichia coli

Biochemistry

39

3149-3155

2000

-

3

-

3

-

2

1

-

1

-

1

-

2

1

-

1

-

3

-

3

-

2

1

-

1

-

2

1

-

1

-

137239

Liao

Crystal structures of the phot ...

Tetradesmus obliquus

Nat. Struct. Biol.

7

749-753

2000

-

1

-

1

-

2

-

2

1

-

1

-

1

-

2

1

-

652088

Spetea

GTP enhances the degradation o ...

Pisum sativum, Spinacia oleracea

J. Biol. Chem.

275

7205-7211

2000

4

-

2

-

2

-

4

-

2

-

653515

Ivleva

The carboxyl-terminal extensio ...

Synechocystis sp.

Plant Physiol.

124

1403-1412

2000

-

3

-

1

-

1

-

137253

Spetea

GTP bound to chloroplast thyla ...

Spinacia oleracea

Proc. Natl. Acad. Sci. USA

96

6547-6552

1999

1

-

2

-

2

-

1

-

1

-

1

-

2

-

1

-

1

-

137237

Keiler

-

Tsp protease ...

Bacillus subtilis, Bartonella bacilliformis, Escherichia coli, Haemophilus influenzae, Neisseria gonorrhoeae

Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press

460-461

1998

-

1

-

6

-

5

-

11

-

1

-

6

-

11

-

137250

Yamamoto

Competitive inhibition analysi ...

Spinacia oleracea

FEBS Lett.

430

261-265

1998

-

1

-

1

-

1

-

137252

Chaal

Characterization of a cDNA enc ...

Arabidopsis thaliana

J. Biol. Chem.

273

689-692

1998

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

137248

Trost

The D1 C-terminal processing p ...

Tetradesmus obliquus

J. Biol. Chem.

272

20348-20356

1997

-

1

-

2

-

3

-

1

-

3

-

1

-

2

-

1

-

3

-

137240

Keiler

Sequence determinants of C-ter ...

Escherichia coli

J. Biol. Chem.

271

2589-2593

1996

-

1

-

1

-

2

-

1

-

8

-

1

-

1

-

1

-

8

-

137245

Inagaki

Carboxy-terminal processing pr ...

Spinacia oleracea

Plant Mol. Biol.

30

39-50

1996

-

1

-

1

-

5

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

137241

Keiler

C-terminal specific protein de ...

Escherichia coli

Protein Sci.

4

1507-1515

1995

-

2

-

2

1

-

2

-

5

1

-

2

-

2

-

2

1

-

5

1

-

2

-

137242

Keiler

Identification of active site ...

Escherichia coli

J. Biol. Chem.

270

28864-28868

1995

-

23

-

1

-

1

-

2

-

23

-

1

-

2

-

137251

Taguchi

Recognition of the structure a ...

Spinacia oleracea

J. Biol. Chem.

270

10711-10716

1995

-

1

-

2

-

2

-

1

-

2

-

137244

Shestakov

Molecular cloning and characte ...

Synechocystis sp.

J. Biol. Chem.

269

19354-19359

1994

-

3

-

1

-

6

-

1

-

3

-

1

-

1

-

137243

Silber

Tsp: a tail-specific protease ...

Escherichia coli

Proc. Natl. Acad. Sci. USA

89

295-299

1992

-

1

-

3

-

3

1

-

4

-

1

-

1

-

3

-

3

1

-

1

-

137249

Bowyeer

Carboxyl-terminal processing o ...

Pisum sativum, Tetradesmus obliquus

J. Biol. Chem.

267

5424-5433

1992

-

3

-

2

-

1

-

2

-

2

-

1

-

2

1

-

2

-

3

-

2

-

1

-

2

-

1

-

2

1

-

2

-

CtpB assembles a gated protease tunnel regulating cell-cell signaling during spore formation in Bacillus subtilis

Data extracted from this reference: