Application | Comment | Organism |
---|---|---|
agriculture | potential target for the discovery of a general broad-spectrum herbicide | Spinacia oleracea |
Cloned (Comment) | Organism |
---|---|
overexpressed in Arabidopsis thaliana, expressed as His-tag fusion protein in Escherichia coli | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | complex inhibitors belonging to the substance classes rhodanine dimer, ketoheterocycles, CF3 peptides, and imidazoles were studied, application of inhibitors to the leaves of Arabidopsis thaliana causes lesions, over expression of CtpA results in enhanced resistance to inhibitor treatment | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-D1 protein + H2O | Spinacia oleracea | catalyzes the conversion of the nascent pre-D1 (pD1) protein into the active form of D1 by cleaving the 9 C-terminal residues in spinach, required to maintain the function of the photosystem II complex | D1 protein + peptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially purified from spinach, recombinant protein from Escherichia coli using His-tag | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
F-24 peptide + H2O | peptide substrate comprising the 24 C-terminal residues of spinach pre-D1 protein, N-terminally fluorescence-labeled with a fluorescein isothiocyanate group | Spinacia oleracea | ? | - |
? | |
pre-D1 protein + H2O | catalyzes the conversion of the nascent pre-D1 (pD1) protein into the active form of D1 by cleaving the 9 C-terminal residues in spinach, required to maintain the function of the photosystem II complex | Spinacia oleracea | D1 protein + peptide | - |
? | |
SP-4 peptide + H2O | peptide substrate comprising the 16 C-terminal residues of spinach pre-D1 protein with an inserted Lys between position 6 and 7 from the C-terminus, the Lys is fluorescence-labeled with a fluorescein isothiocyanate group at the epsilon-amino function, N-terminally biotin-labeled | Spinacia oleracea | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carboxyterminal processing protease of D1 protein | - |
Spinacia oleracea |
CtpA | - |
Spinacia oleracea |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
IC values in the micromolar range found for the effective inhibitors | Spinacia oleracea | additional information |