BRENDA - Enzyme Database show
show all sequences of 3.4.21.102

The carboxyterminal processing protease of D1 protein: Expression, purification and enzymology of the recombinant and native spinach proteins

Fabbri, B.J.; Duff, S.M.G.; Remsen, E.E.; Chen, Y.C.S.; Anderson, J.C.; CaJacob, C.A.; Pest Manag. Sci. 61, 682-690 (2005)

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
the enzyme is a target for general broad-spectrum herbicides
Spinacia oleracea
drug development
the enzyme is a target for development of herbicides
Spinacia oleracea
Cloned(Commentary)
Commentary
Organism
gene ctpA, expression of His-tagged CtpA in Escherichia coli mainly in inclusion bodies
Spinacia oleracea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetics of recombinant and native enzymes using two different assay methods, overview
Spinacia oleracea
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Spinacia oleracea
9507
-
thylakoid membrane
-
Spinacia oleracea
42651
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
recombinant enzyme, gel filtration
Spinacia oleracea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D1 protein + H2O
Spinacia oleracea
C-terminal processing of D1 protein
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Spinacia oleracea
Q41376
precursor
-
Purification (Commentary)
Commentary
Organism
native enzyme from leaves by anion exchange chromatography, hydroxyapatite chromatography, and gel filtration, refolded recombinant His-tagged CtpA by nickel affinity chromatography with a poor yield of active enzyme
Spinacia oleracea
Renatured (Commentary)
Commentary
Organism
solubilization from inclusion bodies and refolding of recombinant His-tagged CtpA
Spinacia oleracea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
development of a high-throughput screening assay method on microtiter plates
Spinacia oleracea
0.000007
0.000015
purified recombinant CtpA, substrates P-24 or F-24 peptides
Spinacia oleracea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D1 protein + H2O
C-terminal processing of D1 protein
670483
Spinacia oleracea
?
-
-
-
?
D1 protein + H2O
substrate from spinach
670483
Spinacia oleracea
?
-
-
-
?
F-24 peptide + H2O
peptide substrate comprising the 24 C-terminal residues of spinach pre-D1 protein, N-terminally fluorescence-labeled with a fluorescein isothiocyanate group
670483
Spinacia oleracea
?
-
-
-
?
P-24 peptide + H2O
peptide substrate comprising the 24 C-terminal residues of spinach pre-D1 protein
670483
Spinacia oleracea
?
-
-
-
?
SP-4 peptide + H2O
peptide substrate comprising the 16 C-terminal residues of spinach pre-D1 protein with an inserted Lys between position 6 and 7 from the C-terminus, the Lys is fluorescence-labeled with a fluorescein isothiocyanate group at the epsilon-amino function, N-terminally biotin-labeled
670483
Spinacia oleracea
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 50000, recombinant enzyme, SDS-PAGE and light scattering
Spinacia oleracea
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Spinacia oleracea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Spinacia oleracea
Application (protein specific)
Application
Commentary
Organism
agriculture
the enzyme is a target for general broad-spectrum herbicides
Spinacia oleracea
drug development
the enzyme is a target for development of herbicides
Spinacia oleracea
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ctpA, expression of His-tagged CtpA in Escherichia coli mainly in inclusion bodies
Spinacia oleracea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetics of recombinant and native enzymes using two different assay methods, overview
Spinacia oleracea
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Spinacia oleracea
9507
-
thylakoid membrane
-
Spinacia oleracea
42651
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
recombinant enzyme, gel filtration
Spinacia oleracea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D1 protein + H2O
Spinacia oleracea
C-terminal processing of D1 protein
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme from leaves by anion exchange chromatography, hydroxyapatite chromatography, and gel filtration, refolded recombinant His-tagged CtpA by nickel affinity chromatography with a poor yield of active enzyme
Spinacia oleracea
Renatured (Commentary) (protein specific)
Commentary
Organism
solubilization from inclusion bodies and refolding of recombinant His-tagged CtpA
Spinacia oleracea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
development of a high-throughput screening assay method on microtiter plates
Spinacia oleracea
0.000007
0.000015
purified recombinant CtpA, substrates P-24 or F-24 peptides
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D1 protein + H2O
C-terminal processing of D1 protein
670483
Spinacia oleracea
?
-
-
-
?
D1 protein + H2O
substrate from spinach
670483
Spinacia oleracea
?
-
-
-
?
F-24 peptide + H2O
peptide substrate comprising the 24 C-terminal residues of spinach pre-D1 protein, N-terminally fluorescence-labeled with a fluorescein isothiocyanate group
670483
Spinacia oleracea
?
-
-
-
?
P-24 peptide + H2O
peptide substrate comprising the 24 C-terminal residues of spinach pre-D1 protein
670483
Spinacia oleracea
?
-
-
-
?
SP-4 peptide + H2O
peptide substrate comprising the 16 C-terminal residues of spinach pre-D1 protein with an inserted Lys between position 6 and 7 from the C-terminus, the Lys is fluorescence-labeled with a fluorescein isothiocyanate group at the epsilon-amino function, N-terminally biotin-labeled
670483
Spinacia oleracea
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 50000, recombinant enzyme, SDS-PAGE and light scattering
Spinacia oleracea
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Spinacia oleracea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Spinacia oleracea
Other publictions for EC 3.4.21.102
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732180
Wegener
An atypical psbA gene encodes ...
Cyanothece sp. ATCC 51142
J. Biol. Chem.
290
3764-3774
2015
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1
1
1
1
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732467
Carroll
The lone S41 family C-terminal ...
Staphylococcus aureus
Microbiology
160
1737-1748
2014
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2
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6
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1
1
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732544
Deng
The periplasmic PDZ domain-con ...
Xanthomonas oryzae pv. oryzae
Mol. Plant Microbe Interact.
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101-112
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-
-
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1
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4
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1
1
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731635
Mastny
CtpB assembles a gated proteas ...
Bacillus subtilis
Cell
155
647-658
2013
1
-
1
1
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-
-
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1
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1
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1
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2
1
1
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1
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2
1
1
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1
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3
3
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731957
Hernandez
Increased bile resistance in S ...
Salmonella enterica, Salmonella enterica SL1344
Int. Microbiol.
16
87-92
2013
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2
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1
1
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732822
Che
C-terminal processing of react ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
110
16247-16252
2013
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1
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1
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1
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1
1
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731328
Yokono
Alterations in photosynthetic ...
Synechocystis sp.
Biochim. Biophys. Acta
1817
754-759
2012
-
-
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1
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5
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732046
Sugiura
Environment of TyrZ in photosy ...
Thermosynechococcus elongatus
J. Biol. Chem.
287
13336-13347
2012
-
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1
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1
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717610
Hoge
The subcellular localization o ...
Pseudomonas aeruginosa
FEMS Microbiol. Lett.
316
23-30
2011
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1
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2
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1
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5
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1
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717653
Zhang
Screening for inhibitors of pl ...
Spinacia oleracea
Hybridoma
30
223-227
2011
-
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1
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1
1
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1
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1
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1
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718206
Yue
A rice stromal processing pept ...
Oryza sativa Indica Group
Plant Cell Physiol.
51
475-485
2010
-
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1
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2
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1
4
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2
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1
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718260
Zou
Expression of a serine proteas ...
Clonostachys rosea, Clonostachys rosea 611
PLoS ONE
5
e13386
2010
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2
1
1
2
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710331
Booij-James
Nitric oxide donor-mediated in ...
Landoltia punctata
Planta
229
1347-1352
2009
-
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1
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4
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679911
Gilbert
Mutagenesis of the carboxy ter ...
Rhizobium leguminosarum
FEMS Microbiol. Lett.
272
65-74
2007
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1
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682255
Duff
-
The carboxyterminal processing ...
Spinacia oleracea
Pestic. Biochem. Physiol.
88
1-13
2007
-
1
1
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1
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1
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1
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1
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669103
Bandara
Carboxyl-terminal protease reg ...
Brucella suis bv. 1
J. Bacteriol.
187
5767-5775
2005
-
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1
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1
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2
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1
1
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10
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2
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1
1
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1
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1
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2
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1
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2
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1
1
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670159
Reiling
Prc protease promotes mucoidy ...
Pseudomonas aeruginosa
Microbiology
151
2251-2261
2005
-
-
1
-
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1
-
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1
-
6
-
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2
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1
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1
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1
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2
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-
670483
Fabbri
The carboxyterminal processing ...
Spinacia oleracea
Pest Manag. Sci.
61
682-690
2005
-
2
1
-
-
-
-
1
2
-
1
1
-
3
-
-
1
-
1
1
2
-
5
1
1
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1
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2
1
-
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1
2
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1
1
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1
1
1
2
-
5
1
1
-
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1
-
-
-
-
-
-
-
-
-
670503
Richter
-
Function of the stromal proces ...
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Substrate recognition through ...
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GTP enhances the degradation o ...
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The carboxyl-terminal extensio ...
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Spetea
GTP bound to chloroplast thyla ...
Spinacia oleracea
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137237
Keiler
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Tsp protease ...
Bacillus subtilis, Bartonella bacilliformis, Escherichia coli, Haemophilus influenzae, Neisseria gonorrhoeae
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Carboxy-terminal processing pr ...
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Identification of active site ...
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Recognition of the structure a ...
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Silber
Tsp: a tail-specific protease ...
Escherichia coli
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Carboxyl-terminal processing o ...
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