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Literature summary for 3.4.21.101 extracted from

  • Oda, K.
    Sedolisin-B (2004), Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press, 2, 1888-1889.
No PubMed abstract available
Search for more literature for 3.4.21.101

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Xanthomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
isovaleryl-tyrosyl-leucyl-tyrosinal tyrostatin Xanthomonas sp.
additional information no inhibition by pepstatin, 2,3-diamino naphthalene and 1,2-Epoxy-3-(p-nitrophenoxy)propane Xanthomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
 1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol pH3.5, 37°C Xanthomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 2 * 41000, SDS-PAGE Xanthomonas sp.
84000
-
 recombinant protein from Escherichia coli Xanthomonas sp.

Organism

Organism UniProt Comment Textmining
Xanthomonas sp. Q60106 T-22
-
Xanthomonas sp. T-22 Q60106 T-22
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acid-denatured hemoglobin + H2O
-
 Xanthomonas sp. ?
-
 ?
Acid-denatured hemoglobin + H2O
-
 Xanthomonas sp. T-22 ?
-
 ?
casein + H2O
-
 Xanthomonas sp. ?
-
 ?
casein + H2O
-
 Xanthomonas sp. T-22 ?
-
 ?
Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu + H2O pH 3.5, 37°C Xanthomonas sp. Lys-Pro-Ala-Asp-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu + H2O pH 3.5, 37°C Xanthomonas sp. T-22 Lys-Pro-Ala-Asp-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
[protein]-3-O-(alpha-D-GlcNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine + H2O
-
 Xanthomonas sp. ?
-
 ?
[protein]-3-O-(alpha-D-GlcNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine + H2O
-
 Xanthomonas sp. T-22 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Xanthomonas sp.

Synonyms

Synonyms Comment Organism
sedolisin-B
-
 Xanthomonas sp.
Xanthomonas serine-carboxyl peptidase
-
 Xanthomonas sp.
XSCO
-
 Xanthomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
52.2
-
 1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol pH3.5, 37°C Xanthomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7
-
 for casein digestion Xanthomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000021
-
 isovaleryl-tyrosyl-leucyl-tyrosinal
-
 Xanthomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Xanthomonas sp.
-
-
 4