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Literature summary for 3.4.21.101 extracted from

  • Oyama, H.; Abe, S.I.; Ushiyama, S.; Takahashi, S.; Oda, K.
    Identification of catalytic residues of pepstatin-insensitive carboxyl proteinases from prokaryotes by site-directed mutagenesis (1999), J. Biol. Chem., 274, 27815-27822.
    View publication on PubMed
Search for more literature for 3.4.21.101

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Xanthomonas sp.

Protein Variants

Protein Variants Comment Organism
D348A production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected Xanthomonas sp.
DELTA192 pro-region mutant enzyme Xanthomonas sp.
S169A production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected Xanthomonas sp.
S79A production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected Xanthomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0058
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme DELTA192 Xanthomonas sp.
0.0111
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme E230A Xanthomonas sp.
0.0188
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme E235A Xanthomonas sp.

Organism

Organism UniProt Comment Textmining
Xanthomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mutant enzymes S79A, S169A and D348A Xanthomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Xanthomonas sp. ?
-
 ?
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
-
 Xanthomonas sp. Lys-Pro-Ala-Leu-Phe + p-nitrophenylalanine-Arg-Leu
-
 ?

Synonyms

Synonyms Comment Organism
XCP
-
 Xanthomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme E235A Xanthomonas sp.
0.9
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme DELTA192 Xanthomonas sp.
21.8
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme E230A Xanthomonas sp.
34.9
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme E235A Xanthomonas sp.
68.4
-
 Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu pH 3.5, 37°C, mutant enzyme DELTA192 Xanthomonas sp.