Literature summary for 3.4.19.9 extracted from

Akhtar, T.A.; McQuinn, R.P.; Naponelli, V.; Gregory, J.F.; Giovannoni, J.J.; Hanson, A.D.
Tomato gamma-glutamylhydrolases: expression, characterization, and evidence for heterodimer formation (2008), Plant Physiol., 148, 775-785.

Search for more literature for 3.4.19.9

Cloned(Commentary)

Cloned (Comment)	Organism
when coexpressed in Escherichia coli, homodimer is catalytically inactive. LeGGH3 forms heterodimers with LeGGH1 or LeGGH2 that has one-half the activity of the matching homodimer	Solanum lycopersicum
when coexpressed in Escherichia coli, LeGGH1 forms heterodimers with an intermediate bond cleavage preference	Solanum lycopersicum
when coexpressed in Escherichia coli, LeGGH2 forms heterodimers with an intermediate bond cleavage preference	Solanum lycopersicum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00098	-	p-aminobenzoylpentaglutamate	-	Solanum lycopersicum
0.0012	-	folic acid pentaglutamate	-	Solanum lycopersicum
0.00131	-	p-aminobenzoylpentaglutamate	-	Solanum lycopersicum
0.00138	-	folic acid pentaglutamate	-	Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36300	-	calculated from sequence	Solanum lycopersicum
36400	-	calculated from sequence	Solanum lycopersicum
36700	-	calculated from sequence	Solanum lycopersicum
39000	-	SDS-PAGE	Solanum lycopersicum
40000	-	SDS-PAGE	Solanum lycopersicum
42000	-	SDS-PAGE	Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
folic acid pentaglutamate + H2O	Solanum lycopersicum	PteGlu5	?	-	?
additional information	Solanum lycopersicum	folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase	?	-	?
additional information	Solanum lycopersicum	folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase. Cleaves PteGlu5 more efficiently than p-aminobenzoylpentaglutamate	?	-	?
additional information	Solanum lycopersicum	folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase. Cleaves PteGlu5 more efficiently than pABAGlu5	?	-	?
p-aminobenzoylpentaglutamate + H2O	Solanum lycopersicum	pABAGlu5	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Solanum lycopersicum	B2Z9Y3	Ailsa Craig	-
Solanum lycopersicum	B2Z9Y4	Ailsa Craig	-
Solanum lycopersicum	B2Z9Y5	Ailsa Craig	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant proteins are purified by Ni2+ affinity chromatography	Solanum lycopersicum
recombinant proteins are purified by Ni2+ affinity chromatography; in the case of LeGGH1, an additional cation exchange step is required	Solanum lycopersicum

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower bud	-	Solanum lycopersicum	-
fruit	and all other organs	Solanum lycopersicum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
folic acid pentaglutamate + H2O	PteGlu5	Solanum lycopersicum	?	-	?
additional information	folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase	Solanum lycopersicum	?	-	?
additional information	folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase. Cleaves PteGlu5 more efficiently than p-aminobenzoylpentaglutamate	Solanum lycopersicum	?	-	?
additional information	folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase. Cleaves PteGlu5 more efficiently than pABAGlu5	Solanum lycopersicum	?	-	?
p-aminobenzoylpentaglutamate + H2O	pABAGlu5	Solanum lycopersicum	?	-	?

Subunits

Subunits	Comment	Organism
heterodimer	-	Solanum lycopersicum
heterodimer	the homodimer is catalytically inactive	Solanum lycopersicum

Synonyms

Synonyms	Comment	Organism
gamma-glutamyl hydrolase	-	Solanum lycopersicum
GGH	-	Solanum lycopersicum
LeGGH1	-	Solanum lycopersicum
LeGGH2	expressed in fruit all other organs	Solanum lycopersicum
LeGGH3	expressed mainly in flower buds, the homodimer is catalytically inactive	Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.96	-	p-aminobenzoylpentaglutamate	-	Solanum lycopersicum
1.08	-	p-aminobenzoylpentaglutamate	-	Solanum lycopersicum
1.69	-	folic acid pentaglutamate	-	Solanum lycopersicum
2.13	-	folic acid pentaglutamate	-	Solanum lycopersicum

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Release 2023.1 (January 2023)