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Literature summary for 3.4.19.9 extracted from
- Optimization of the trienzyme extraction for the microbiological assay of folate in vegetables (2007), J. Agric. Food Chem., 55, 3884-3888.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | trienzyme extraction is a combined enzymatic digestion by Pronase, alpha-amylase, and conjugase, i.e. gamma-glutamyl hydrolase, to liberate the carbohydrate and protein-bound folates from food matrices prior to total folate analysis by microbiological assay using Lactobacillus casei ssp. rhamnosus, ATCC 7469, determine the contribution of the enzymes to the growth response, the extraction method is used in AOAC Official Method 2004.05 for the folate analysis of cereal foods | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Gallus gallus | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| conjugase | - |
Gallus gallus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Gallus gallus |
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