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Literature summary for 3.4.19.9 extracted from
- Site-directed mutagenesis establishes cysteine-110 as essential for enzyme activity in human gamma-glutamyl hydrolase (1999), Biochem. J., 343, 551-555.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type and mutant enymes C19A, C110A, C124A and C290A are expressed in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C124A | Km-value for methotrexate diglutamate is not significantly different from the Km-value of the wild-type enzyme. Specific activity is significantly lower than that of the wild-type enzyme, but the mutant protein has a higher amount of contaminating protein | Homo sapiens |
| C19A | Km-value for methotrexate diglutamate is not significantly different from the Km-value of the wild-type enzyme. Specific activity is significantly lower than that of the wild-type enzyme, but the mutant protein has a higher amount of contaminating protein | Homo sapiens |
| C290A | Km-value for methotrexate diglutamate is not significantly different from the Km-value of the wild-type enzyme | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0222 | - |
methotrexate diglutamate | mutant enzyme C19A | Homo sapiens | |
| 0.0231 | - |
methotrexate diglutamate | mutant enzyme C290A | Homo sapiens | |
| 0.0303 | - |
methotrexate diglutamate | mutant enzyme C124A | Homo sapiens | |
| 0.049 | - |
methotrexate diglutamate | wild-type enzyme | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pteroylpolyglutamate + H2O | Homo sapiens | key enzyme in the maintenance of cellular folylpolyglutamate concentration | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methotrexate diglutamate + H2O | - |
Homo sapiens | ? | - |
? | |
| pteroylpolyglutamate + H2O | key enzyme in the maintenance of cellular folylpolyglutamate concentration | Homo sapiens | ? | - |
? | |
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