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Literature summary for 3.4.19.9 extracted from
- Intracellular pteroylpolyglutamate hydrolase from human jejunal mucosa. Isolation and characterization (1986), J. Biol. Chem., 261, 13551-13555.
General Stability
| General Stability | Organism |
|---|---|
| substrate protects | Homo sapiens |
| unaffected by dialysis against 1 mM EDTA | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| gamma-diglutamate | - |
Homo sapiens |  |
| additional information | no inhibition by alpha-diglutamic acid; no inhibition by alpha-triglutamic acid; no inhibition by glutamic acid | Homo sapiens | |
| p-aminobenzoylglutamate | slight | Homo sapiens | |
| Pterine | - |
Homo sapiens | |
| pteroyldiglutamate | hydrolysis of pteroyltriglutamate | Homo sapiens | |
| Pteroylheptaglutamate | hydrolysis of pteroyltriglutamate | Homo sapiens | |
| Zn2+ | 0.1 mM zinc acetate, weak | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
Pteroyltriglutamate | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Homo sapiens | 5622 | - |
| lysosome | - |
Homo sapiens | 5764 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
human, gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| jejunum | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the intracellular enzyme shows the greatest affinity for the complete folic acid molecule with longer glutamate chains | Homo sapiens | ? | - |
? | |
| additional information | the intracellular enzyme cleaves both terminal and internal gamma-glutamate linkages, in contrast the brush-border enzyme catalyzes the hydrolysis of only terminal gamma-glutamate linkages | Homo sapiens | ? | - |
? | |
| pteroyltriglutamate + H2O | - |
Homo sapiens | ? | - |
? | |
| pteroyltriglutamate + H2O | - |
Homo sapiens | pteroylglutamate + glutamate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
unstable at pH 4.5, protective effect of the substrate | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Homo sapiens |
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