Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | weak | Myrothecium sp. | |
EDTA | no inhibition | Myrothecium sp. | |
additional information | no inhibition by competitive inhibitors of serine and cysteine proteinases; no inhibition by phosphoramidon | Myrothecium sp. | |
PCMB | - |
Myrothecium sp. | |
Phenylmethylsulfonylfluoride | no inhibition | Myrothecium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
gel filtration | Myrothecium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Myrothecium sp. | - |
TM-4222 | - |
Myrothecium sp. TM-4222 | - |
TM-4222 | - |
Purification (Comment) | Organism |
---|---|
- |
Myrothecium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Myrothecium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Myrothecium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly-Glu + H2O | enzyme is specific for poly(gamma-glutamic) acid, but not for other gamma-glutamyl peptides or amides | Myrothecium sp. | ? | endo-type specificity, 38% of the original poly-Glu with an average MW of 500000 is converted to smaller peptides, and then depolymerized to a mixture of gamma-oligopeptides which consist of only L-glutamic acid, L-glutamic acid monomer is negligible in the reaction mixture, the remaining 62% of poly(gamma-glutamic acid) are resistant to the enzyme action | ? | |
poly-Glu + H2O | enzyme is specific for poly(gamma-glutamic) acid, but not for other gamma-glutamyl peptides or amides | Myrothecium sp. TM-4222 | ? | endo-type specificity, 38% of the original poly-Glu with an average MW of 500000 is converted to smaller peptides, and then depolymerized to a mixture of gamma-oligopeptides which consist of only L-glutamic acid, L-glutamic acid monomer is negligible in the reaction mixture, the remaining 62% of poly(gamma-glutamic acid) are resistant to the enzyme action | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Myrothecium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to, 1 h, pH 5.0 | Myrothecium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Myrothecium sp. |
7.8 | - |
- |
Myrothecium sp. |