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Literature summary for 3.4.19.7 extracted from
- Isolation and purification of N-formylmethionine aminopeptidase from rat intestine (1992), Biochim. Biophys. Acta, 1119, 275-280.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Benzyl succinate | - |
Rattus norvegicus |  |
| Cd2+ | - |
Rattus norvegicus | |
| Cu2+ | markedly | Rattus norvegicus | |
| Fe2+ | not | Rattus norvegicus | |
| Hg2+ | - |
Rattus norvegicus | |
| additional information | 2-mercaptoethanol; DTT; N3-; not: EDTA | Rattus norvegicus | |
| phenylmethanesulfonyl fluoride | weak | Rattus norvegicus | |
| Zn2+ | moderate | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
Acetyl-Met-Leu | - |
Rattus norvegicus | |
| 0.04 | - |
N-Formyl-Met-Leu | - |
Rattus norvegicus | |
| 0.06 | - |
N-Formyl-Met-Val | - |
Rattus norvegicus | |
| 0.16 | - |
Acetyl-Met-Leu-Phe | - |
Rattus norvegicus | |
| 0.18 | - |
N-Formyl-Met-Leu-Phe | - |
Rattus norvegicus | |
| 0.22 | - |
N-Formyl-Met-Leu-Tyr | - |
Rattus norvegicus | |
| 0.23 | - |
Formylnorleucine-Leu-Phe | - |
Rattus norvegicus | |
| 0.63 | - |
N-Formyl-Met-Phe | - |
Rattus norvegicus | |
| 0.71 | - |
N-Formyl-Met-Trp | - |
Rattus norvegicus | |
| 1.7 | - |
N-Formyl-Met 2-naphthylamide | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | activates | Rattus norvegicus | |
| Cl- | maximal activity at 1 M | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-Formylmethionyl oligopeptides + H2O | Rattus norvegicus | the presence of this enzyme in intestinal mucosa can prevent absorption of intact bioactive potent inflammatory N-formylmethionyl oligopeptides produced by commensal bacteria in the gut lumen | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| intestine | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gut | lowest level in oesophagus and stomach and a shallow diminishing gradient from proximal small intestine to distal colon and rectum | Rattus norvegicus | - |
| intestine | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.86 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Acetyl-Met-Leu + H2O | - |
Rattus norvegicus | ? | - |
? | |
| Acetyl-Met-Leu-Phe + H2O | - |
Rattus norvegicus | ? | - |
? | |
| Formylnorleucine-Leu-Phe + H2O | - |
Rattus norvegicus | ? | - |
? | |
| N-Formyl-Met 2-naphthylamide + H2O | - |
Rattus norvegicus | N-Formyl-Met + 2-naphthylamine | - |
? | |
| N-Formyl-Met-Leu + H2O | - |
Rattus norvegicus | N-Formyl-Met + Leu | - |
? | |
| N-Formyl-Met-Leu-Phe + H2O | - |
Rattus norvegicus | N-Formyl-Met + Leu-Phe | - |
? | |
| N-Formyl-Met-Leu-Tyr + H2O | - |
Rattus norvegicus | N-Formyl-Met + Leu-Tyr | - |
? | |
| N-Formyl-Met-Phe + H2O | - |
Rattus norvegicus | N-Formyl-Met + Phe | - |
? | |
| N-Formyl-Met-Trp + H2O | - |
Rattus norvegicus | N-Formyl-Met + Trp | - |
? | |
| N-Formyl-Met-Val + H2O | - |
Rattus norvegicus | N-Formyl-Met + Val | - |
? | |
| N-Formylmethionyl oligopeptides + H2O | the presence of this enzyme in intestinal mucosa can prevent absorption of intact bioactive potent inflammatory N-formylmethionyl oligopeptides produced by commensal bacteria in the gut lumen | Rattus norvegicus | ? | - |
? | |
| Natural formylmethionine oligopeptide from E. coli culture supernatants + H2O | loss of bioactivity and immuno-reactivity | Rattus norvegicus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.17 | - |
N-Formyl-Met-Leu | - |
Rattus norvegicus | |
| 5.45 | - |
Acetyl-Met-Leu | - |
Rattus norvegicus | |
| 5.94 | - |
N-Formyl-Met-Val | - |
Rattus norvegicus | |
| 12.3 | - |
N-Formyl-Met-Leu-Phe | - |
Rattus norvegicus | |
| 15.5 | - |
N-Formyl-Met-Leu-Tyr | - |
Rattus norvegicus | |
| 27.1 | - |
Acetyl-Met-Leu-Phe | - |
Rattus norvegicus | |
| 31.3 | - |
N-Formyl-Met-Trp | - |
Rattus norvegicus | |
| 70.2 | - |
N-Formyl-Met-Phe | - |
Rattus norvegicus | |
| 76.4 | - |
N-Formyl-Met 2-naphthylamide | - |
Rattus norvegicus | |
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