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Literature summary for 3.4.19.5 extracted from

  • Park, S.H.; Lee, C.W.; Lee, S.G.; Shin, S.C.; Kim, H.J.; Park, H.; Lee, J.H.
    Crystal structure and functional characterization of an isoaspartyl dipeptidase (CpsIadA) from Colwellia psychrerythraea strain 34H (2017), PLoS ONE, 12, e0181705 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.19.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Colwellia psychrerythraea

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of the isoaspartyl dipeptidase from Colwellia psychrerythraea, both ligand-free and that complexed with beta-isoaspartyl lysine, at 1.85 A and 2.33 A resolution, respectively. In both structures, the enzyme forms an octamer with two Zn2+ ions in the active site Colwellia psychrerythraea

Protein Variants

Protein Variants Comment Organism
E166A the mutant exhibits a complete loss of activity and a considerable decrease in melting temperature Colwellia psychrerythraea
E166K the mutant exhibits a complete loss of activity and a considerable decrease in melting temperature Colwellia psychrerythraea
E80Q the mutant has no catalytic activity toward beta-Asp-Leu, but its CD spectra and denaturation temperature are similar to wild-type, indicating that this mutation affects catalytic activity but not the overall folding and integrity of the enzyme Colwellia psychrerythraea
Y140F the mutant has a significant reduction rate of catalysis confirming that this tyrosine residue is important for enzymatic catalysis. It shows less than 10% catalytic activity toward beta-Asp-Leu and the CD spectrum is not significantly different to the wild-type enzyme. Unlike the wild-type enzyme and the other mutants Y140F has a tendency to aggregate during purification and storage, but soluble Y140F is the most thermally stable Colwellia psychrerythraea

General Stability

General Stability Organism
thermostable, probably owing to its octameric structure Colwellia psychrerythraea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.49
-
 beta-Asp-Phe pH 8.0, 30°C Colwellia psychrerythraea
0.71
-
 beta-Asp-Leu pH 8.0, 30°C Colwellia psychrerythraea
0.93
-
 beta-Ala-Ala pH 8.0, 30°C Colwellia psychrerythraea
1.1
-
 beta-Asp-Lys pH 8.0, 30°C Colwellia psychrerythraea
1.2
-
 beta-Asp-Ala pH 8.0, 30°C Colwellia psychrerythraea
4.7
-
 beta-Asp-Gly pH 8.0, 30°C Colwellia psychrerythraea
5.3
-
 beta-Asp-His pH 8.0, 30°C Colwellia psychrerythraea
6.9
-
 alpha-Asp-Leu pH 8.0, 30°C Colwellia psychrerythraea

Organism

Organism UniProt Comment Textmining
Colwellia psychrerythraea Q484B6
-
-
Colwellia psychrerythraea ATCC BAA-681 Q484B6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Colwellia psychrerythraea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-Asp-Leu + H2O
-
 Colwellia psychrerythraea Asp + Leu
-
 ?
beta-Ala-Ala + H2O
-
 Colwellia psychrerythraea Ala + Ala
-
 ?
beta-Asp-Ala + H2O
-
 Colwellia psychrerythraea Asp + Ala
-
 ?
beta-Asp-Ala + H2O
-
 Colwellia psychrerythraea ATCC BAA-681 Asp + Ala
-
 ?
beta-Asp-Gly + H2O
-
 Colwellia psychrerythraea Asp + Gly
-
 ?
beta-Asp-Gly + H2O
-
 Colwellia psychrerythraea ATCC BAA-681 Asp + Gly
-
 ?
beta-Asp-His + H2O
-
 Colwellia psychrerythraea Asp + His
-
 ?
beta-Asp-His + H2O
-
 Colwellia psychrerythraea ATCC BAA-681 Asp + His
-
 ?
beta-Asp-Leu + H2O
-
 Colwellia psychrerythraea Asp + Leu
-
 ?
beta-Asp-Leu + H2O
-
 Colwellia psychrerythraea ATCC BAA-681 Asp + Leu
-
 ?
beta-Asp-Lys + H2O
-
 Colwellia psychrerythraea Asp + Lys
-
 ?
beta-Asp-Lys + H2O
-
 Colwellia psychrerythraea ATCC BAA-681 Asp + Lys
-
 ?
beta-Asp-Phe + H2O
-
 Colwellia psychrerythraea Asp + Phe
-
 ?

Subunits

Subunits Comment Organism
octamer crystallographic data, the enzyme forms an octamer with two Zn2+ ions in the active site Colwellia psychrerythraea

Synonyms

Synonyms Comment Organism
CpsIadA
-
 Colwellia psychrerythraea
isoaspartyl aminopeptidase/asparaginase
-
 Colwellia psychrerythraea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Colwellia psychrerythraea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 60% of maximal activity, 50°C: about 70% of maximal activity Colwellia psychrerythraea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 completely inactivated Colwellia psychrerythraea
66
-
 denaturation temperature, mutant enzyme E166K Colwellia psychrerythraea
71
-
 denaturation temperature, mutant enzyme E166A Colwellia psychrerythraea
81
-
 denaturation temperature, wild-type enzyme Colwellia psychrerythraea
85
-
 denaturation temperature, mutant enzyme E80Q Colwellia psychrerythraea
89
-
 denaturation temperature, mutant enzyme Y140F Colwellia psychrerythraea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
74
-
 beta-Asp-His pH 8.0, 30°C Colwellia psychrerythraea
89
-
 alpha-Asp-Leu pH 8.0, 30°C Colwellia psychrerythraea
145
-
 beta-Asp-Phe pH 8.0, 30°C Colwellia psychrerythraea
164
-
 beta-Asp-Leu pH 8.0, 30°C Colwellia psychrerythraea
166
-
 beta-Asp-Ala pH 8.0, 30°C Colwellia psychrerythraea
181
-
 beta-Asp-Gly pH 8.0, 30°C Colwellia psychrerythraea
256
-
 beta-Asp-Lys pH 8.0, 30°C Colwellia psychrerythraea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Colwellia psychrerythraea

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 pH 7.5: about 35% of maximal activity, pH 9.0: about 20% of maximal activity Colwellia psychrerythraea

General Information

General Information Comment Organism
additional information the enzyme catalyzes the hydrolysis of an isoaspartyl dipeptide-like moiety, which can be inappropriately formed in proteins, between the beta-carboxyl group side chain of Asp and the amino group of the following amino acid Colwellia psychrerythraea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13
-
 alpha-Asp-Leu pH 8.0, 30°C Colwellia psychrerythraea
14
-
 beta-Asp-His pH 8.0, 30°C Colwellia psychrerythraea
38
-
 beta-Asp-Gly pH 8.0, 30°C Colwellia psychrerythraea
140
-
 beta-Asp-Ala pH 8.0, 30°C Colwellia psychrerythraea
230
-
 beta-Asp-Lys pH 8.0, 30°C Colwellia psychrerythraea
230.9
-
 beta-Asp-Leu pH 8.0, 30°C Colwellia psychrerythraea
300
-
 beta-Asp-Phe pH 8.0, 30°C Colwellia psychrerythraea