Literature summary for 3.4.19.13 extracted from

Shibayama, K.; Wachino, J.; Arakawa, Y.; Saidijam, M.; Rutherford, N.; Henderson, P.
Metabolism of glutamine and glutathione via gamma-glutamyltranspeptidase and glutamate transport in Helicobacter pylori: Possible significance in the pathophysiology of the organism (2007), Mol. Microbiol., 64, 396-406.

Search for more literature for 3.4.19.13

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Helicobacter pylori

Inhibitors

Inhibitors	Comment	Organism	Structure
6-diazo-5-oxo-L-norleucine	pretreatment of cells completely abolishes the extracellular hydrolysis of lgutathione and glutamine	Helicobacter pylori

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00133	-	L-glutamine	pH 6.0, 37°C	Helicobacter pylori
0.181	-	L-glutamine	pH 8.0, 37°C	Helicobacter pylori
0.23	-	L-glutamine	pH 7.0, 37°C	Helicobacter pylori
0.52	-	L-glutamine	pH 9.0, 37°C	Helicobacter pylori
0.918	-	glutathione	pH 6.0, 37°C	Helicobacter pylori

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Helicobacter pylori	-	-

Organism

Organism	UniProt	Comment	Textmining
Helicobacter pylori	O25743	enzyme catalyses both gamma-glutamyl transfer, reaction of EC 2.3.2.2, and hydrolysis reactions at the same time	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Helicobacter pylori

Reaction

Reaction	Comment	Organism	Reaction ID
glutathione + H2O = L-cysteinylglycine + L-glutamate	ping-pong kinetics mechanism, in which an acyl enzyme intermediate is formed	Helicobacter pylori	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione + H2O	-	Helicobacter pylori	L-cysteinylglycine + L-glutamate	-	?
L-glutamine + H2O	-	Helicobacter pylori	L-glutamate + NH3	-	?

Synonyms

Synonyms	Comment	Organism
hp1118	-	Helicobacter pylori

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	9.3	hydrolysis activity is optimal and almost constant within	Helicobacter pylori

General Information

General Information	Comment	Organism
physiological function	one of the principle physiological functions of the enzyme is to enable Helicobacter pylori cells to utilize extracellular glutamine and glutathione as a source of glutamate. Helicobacter pylori cells are unable to take up extracellular glutamine and glutathione directly. Instead, these substances are hydrolysed to glutamate by the action of the enzyme outside the cells	Helicobacter pylori

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Release 2023.1 (January 2023)