Cloned (Comment) | Organism |
---|---|
recombinant UCH37 catalytic domain as N-terminally GST-tagged protein is expressed in Escherichia coli BL21(RIPL) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant UCH37 catalytic domain, i.e. UCH37N, selenomethionine-substituted UCH37N, and of mutant C88A, sitting-drop vapor diffusion method, 14 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 1 mM DTT, 20°C, versus a reservoir solution containing 22% PEG 4000, 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, and 3.5% xylitol, microseeding, X-ray diffraction structure determination and analysis at 2.2 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C88A | mutant structure determination and comparison to the wild-type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9Y5K5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally GST-tagged UCH37 catalytic domain from Escherichia coli BL21(RIPL) by glutathione affinity chromatography, proteolytic removal of the GST-tag, and gel filtration | Homo sapiens |
Subunits | Comment | Organism |
---|---|---|
More | the catalytic domain, the UCH-domain, i.e. UCH37N, of UCH37 comprises residues 1-228 residues. UCH37N is composed of a central six-stranded anti-parallel beta-sheet, with seven alpha-helices on either side of the sheet causing it to form a bilobal structure, loop and active site structures, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
UCH-L5 | - |
Homo sapiens |
UCH37 | - |
Homo sapiens |