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Literature summary for 3.4.19.12 extracted from

  • Whitehurst, C.B.; Ning, S.; Bentz, G.L.; Dufour, F.; Gershburg, E.; Shackelford, J.; Langelier, Y.; Pagano, J.S.
    The Epstein-Barr virus (EBV) deubiquitinating enzyme BPLF1 reduces EBV ribonucleotide reductase activity (2009), J. Virol., 83, 4345-4353.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.19.12

Cloned(Commentary)

Cloned (Comment) Organism
BPLF1 N-terminal fragment containing the first 246 amino acids cloned into the pFLAG-CMV-2 vector. Expression of the fragment or mutant in HEK-293T cells Human gammaherpesvirus 4

Protein Variants

Protein Variants Comment Organism
C61S loss of deubiquitinating enzyme activity, does not decrease viral ribonucleotide reductase activity Human gammaherpesvirus 4

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm BPLF1 1-246 is expressed alone or with ribonucleotide reductase 1 or ribonucleotide reductase 2 Human gammaherpesvirus 4 5737
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nucleus BPLF1 1-246 is expressed alone or with ribonucleotide reductase 1 or ribonucleotide reductase 2 Human gammaherpesvirus 4 5634
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Human gammaherpesvirus 4 shRNA decreases mRNA levels and reduces viral genome production. EBV deubiquitinating enzyme BPLF1 interacts with, deubiquitinates, and influences the activity of the Epstein-Barr virus ribonucleotide reductase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Human gammaherpesvirus 4 P03186
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-

Purification (Commentary)

Purification (Comment) Organism
BPLF1 1-246 and BPLF1 C61S purified by immunoprecipitation Human gammaherpesvirus 4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
EBV ribonucleotide reductase is deubiquitinated by BPLF1 1-246 Human gammaherpesvirus 4 ?
-
 ?
additional information shRNA decreases mRNA levels and reduces viral genome production. EBV deubiquitinating enzyme BPLF1 interacts with, deubiquitinates, and influences the activity of the Epstein-Barr virus ribonucleotide reductase Human gammaherpesvirus 4 ?
-
 ?
ubiquitin-AMC BPLF1 1-246 expresses deubiquitinating activity, cleaves ubiquitin chains linked by K48 and K63 Human gammaherpesvirus 4 ?
-
 ?

Synonyms

Synonyms Comment Organism
BPLF1
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 Human gammaherpesvirus 4