Application | Comment | Organism |
---|---|---|
medicine | ubiquitin hydrolase Uch-L1 could be an attractive target for the development of new therapeutic approaches to Alzheimer's disease | Mus musculus |
medicine | Uch-L1 is required for normal synaptic and cognitive function. Uch-L1 activity plays a role in normal contextual fear learning. Transduction of Uch-L1 protein fused to the transduction domain of HIV-transactivator protein restores normal enzymatic activity and synaptic function both in hippocampal slices treated with oligomeric Ab and in the APP/PS1 mouse model of Alzheimers disease. Intraperitoneal injections with the fusion protein improve the retention of contextual learning in APP/PS1 mice over time. Beneficial effect of the Uch-L1 fusion protein is associated with restoration of normal levels of the protein kinase A-regulatory subunit IIa, PKA activity, and cAMP response element binding protein phosphorylation | Mus musculus |
Cloned (Comment) | Organism |
---|---|
recombinant fusion proteins expressed in Escherichia Coli BL21(DE3)pLysS cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C90S | mutant of the Uch-L1 protein fused to the transduction domain of HIV-transactivator protein, has little activity | Mus musculus |
additional information | mutant of the Uch-L1 protein fused to the transduction domain of HIV-transactivator protein with a 57 amino acid deletion (130-186) including the H161 site, is inactive | Mus musculus |
S18Y | mutant of the Uch-L1 protein fused to the transduction domain of HIV-transactivator protein, has similar hydrolase activity than the unmutated fusion protein | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amyloid beta | 30% inhibition | Mus musculus | |
LDN-57444 | reversible, competitive, active site-directed isatin oxime, 70% inhibition with 0.005 mM | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | Uch-L1 protein fused to the transduction domain of HIV-transactivator protein exclusively present in the soluble fraction of hippocampal homogenates | Mus musculus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | ubiquitin hydrolase Uch-L1 rescues beta-amyloid-induced decreases in synaptic function and contextual memory | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
C57/BL6 mice or APP/PS1 mice | - |
Mus musculus | Q9R0P9 | - |
- |
Purification (Comment) | Organism |
---|---|
fusion proteins purified by ProBond purification system | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
hippocampus | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ubiquitin hydrolase Uch-L1 rescues beta-amyloid-induced decreases in synaptic function and contextual memory | Mus musculus | ? | - |
? | |
ubiquitin-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | ubiquitin + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ubiquitin C-terminal hydrolase L1 | - |
Mus musculus |
ubiquitin hydrolase Uch-L1 | - |
Mus musculus |
UCH-L1 | - |
Mus musculus |