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Literature summary for 3.4.19.12 extracted from

  • Nishikawa, K.; Li, H.; Kawamura, R.; Osaka, H.; Wang, Y.L.; Hara, Y.; Hirokawa, T.; Manago, Y.; Amano, T.; Noda, M.; Aoki, S.; Wada, K.
    Alterations of structure and hydrolase activity of parkinsonism-associated human ubiquitin carboxyl-terminal hydrolase L1 variants (2003), Biochem. Biophys. Res. Commun., 304, 176-183.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozyme L1: expression of wild-type and mutants in Escherichia coli DH5alphaPRO as His-tagged proteins Homo sapiens

Protein Variants

Protein Variants Comment Organism
C90S site-directed mutagenesis, isozyme L1, inactive Homo sapiens
C90S exchange of the active site cysteine Homo sapiens
I93M site-directed mutagenesis, isozyme L1, mutant shows increased risk for Parkinson's disease, 45.6% enzyme activity compared to wild-type Homo sapiens
S18Y site-directed mutagenesis, isozyme L1, mutant shows decreased risk for Parkinson's disease, 112.6% enzyme activity compared to wild-type Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
aldehyde product of fatty acid peroxidation i.e. HNE; modifies isozyme L1 at physiological concentrations of 0.01-0.1 mM and reduces enzyme activity, excess N-acetyl-L-cysteine protects Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00011
-
ubiquitin-7-amido-4-methylcoumarin recombinant mutant I93M isozyme L1, pH 7.8, room temperature Homo sapiens
0.00012
-
ubiquitin-7-amido-4-methylcoumarin recombinant wild-type isozyme L1, pH 7.8, room temperature Homo sapiens
0.00014
-
ubiquitin-7-amido-4-methylcoumarin recombinant mutant S18Y isozyme L1, pH 7.8, room temperature Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens increased activity of neuronal isozyme L1 reduces the risk for Parkinson's disease ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P09936 isozyme L1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutants of isozyme L1 expressed in Escherichia coli DH5alphaPRO Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain isozyme L1, adult Homo sapiens
-
neuron isozyme L1 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information increased activity of neuronal isozyme L1 reduces the risk for Parkinson's disease Homo sapiens ?
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O recombinant wild-type isozyme L1 and mutants I93M and S18Y Homo sapiens ubiquitin + 7-amino-4-methylcoumarin
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O fluorogenic synthetic substrate Homo sapiens ubiquitin + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
More secondary structure analysis of wild-type and mutant isozyme L1 by circular dichroism spectroscopy Homo sapiens

Synonyms

Synonyms Comment Organism
UCH
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
room temperature, assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0794
-
ubiquitin-7-amido-4-methylcoumarin recombinant mutant I93M isozyme L1, pH 7.8, room temperature Homo sapiens
0.174
-
ubiquitin-7-amido-4-methylcoumarin recombinant wild-type isozyme L1, pH 7.8, room temperature Homo sapiens
0.196
-
ubiquitin-7-amido-4-methylcoumarin recombinant mutant S18Y isozyme L1, pH 7.8, room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Homo sapiens