Cloned (Comment) | Organism |
---|---|
co-expression of Doa4p with cyk-3 of Caenorhabditis elegans and ubiquitin-beta-galactosidase fusion protein in Escherichia coli | Saccharomyces cerevisiae |
gene cyk-3, chromosome mapping, co-expression of cyk-3 with Doa4p of Saccharomyces cerevisiae and ubiquitin-beta-galactosidase fusion protein in Escherichia coli | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | - |
Saccharomyces cerevisiae |
additional information | gene cyk-3 null mutants are lethal, mutants fail to polarize the actin cytoskeleton, to segregate germline determinants, to assemble an intact cleavage furrow, and are intrinsically defective in osmotic regulation and cytokinesis, the latter defect can be partially rescued by providing osmotic support | Caenorhabditis elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquitin-protein + H2O | Saccharomyces cerevisiae | - |
ubiquitin + protein | - |
? | |
ubiquitin-protein + H2O | Caenorhabditis elegans | enzyme is crucial for polarization of the actin cytoskeleton, to segregate germline determinants, to assemble an intact cleavage furrow, for cytokinesis and to maintain osmotic balance and execute actin-dependent processes in the early embryo | ubiquitin + protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
gene cyk-3, defects are lethal | - |
Saccharomyces cerevisiae | - |
Doa4p ubiquitin isopeptidase | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | early stage | Caenorhabditis elegans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linear ubiquitin-beta-galactosidase + H2O | recombinant fusion protein co-expressed with the enzyme in Escherichia coli | Saccharomyces cerevisiae | ubiquitin + protein | - |
? | |
linear ubiquitin-beta-galactosidase + H2O | recombinant fusion protein co-expressed with the enzyme in Escherichia coli | Caenorhabditis elegans | ubiquitin + protein | - |
? | |
ubiquitin-protein + H2O | - |
Saccharomyces cerevisiae | ubiquitin + protein | - |
? | |
ubiquitin-protein + H2O | enzyme is crucial for polarization of the actin cytoskeleton, to segregate germline determinants, to assemble an intact cleavage furrow, for cytokinesis and to maintain osmotic balance and execute actin-dependent processes in the early embryo | Caenorhabditis elegans | ubiquitin + protein | - |
? |