Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DD-Diaminopimelic acid isomers | - |
Lysinibacillus sphaericus | |
Dicarboxylic D-amino acids | inhibition is a function of length, maximum at n=4 | Lysinibacillus sphaericus | |
meso-diaminopimelic acid isomers | - |
Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
N-acetylmuramoyl-L-Ala-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala | - |
Lysinibacillus sphaericus | |
1.99 | - |
N-acetylglucosamine-N-acetylmuramoyl-L-Ala-D-Glu-[-(L)-meso-diaminopimelic acid-D-Ala]NH2 | immobilized enzyme | Lysinibacillus sphaericus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Lysinibacillus sphaericus | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
NCTC 9602 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | hydrolysis of substrates containing D-Glu-meso-diaminopimelic-acid with free omega-NH2 and omega-COOH groups, the presence or absence of an alpha-amide group on glutamic acid, the second residue of peptides, has no influence on the specificity. N-Substitution of N-terminal L-Ala does not modify the enzymic specificity. An amide group on glutamic acid gives an decrease of 80% of activity | Lysinibacillus sphaericus | ? | - |
? | |
additional information | hydrolysis of substrates containing D-Glu-meso-diaminopimelic-acid with free omega-NH2 and omega-COOH groups, the presence or absence of an alpha-amide group on glutamic acid, the second residue of peptides, has no influence on the specificity. N-Substitution of N-terminal L-Ala does not modify the enzymic specificity. An amide group on glutamic acid gives an decrease of 80% of activity | Lysinibacillus sphaericus 9602 | ? | - |
? | |
N-Acetylmuramoyl-L-Ala-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala + H2O | best substrate | Lysinibacillus sphaericus | N-Acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid-(L)-D-Ala | - |
? | |
N-Acetylmuramoyl-L-Ala-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala + H2O | best substrate | Lysinibacillus sphaericus 9602 | N-Acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid-(L)-D-Ala | - |
? |