Cloned (Comment) | Organism |
---|---|
a truncated mutant of apAPH that lacks the first short alpha-helix at the N-terminal is cloned and expressed in Escherichia coli | Aeropyrum pernix K1 |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method, 2.5 A resolution, space group: P2(1)2(1)2(1), unit cell parameters: a = 63.1 A, b = 102.3 A, c = 163.9 A, truncated mutant of apAPH that lacks the first short alpha-helix at the N-terminal is cloned and expressed in Escherichia coli | Aeropyrum pernix K1 |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-21 | optimal temperature of a truncated mutant of apAPH that lacks the first short alpha-helix at the N-terminal is decreased by 15°C | Aeropyrum pernix K1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix K1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aeropyrum pernix K1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
truncated mutant of apAPH that lacks the first short alpha-helix at the N-terminal | Aeropyrum pernix K1 |
90 | - |
wild-type enzyme | Aeropyrum pernix K1 |