General Stability | Organism |
---|---|
1 M guanidine-HCl, activity is completely abolished. Dialysis results in 15-20% recovery of enzyme activity. In 1 M guanidine HCl the enzyme loses both its secondary and tertiary structures and dissociates into monomers of 70000 Da. Both monomeric and dimeric species are observed after 24 h dialysis of the enzyme denatured with guanidine-HCl. Both the monomer and dimer forms recovered after dialysis are active | Sus scrofa |
8 M urea, 1 h, 25°C, 85% loss of activity. Complete recovery on dialysis or 50fold dilution of the denatured enzyme. The enzyme lost its secondary structure at urea concentrations of 2 M and higher, whereas the tertiary structure is minimally perturbed below 4 M urea | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
N-acetyl-Ala-p-nitroanilide | pH 7.5 | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
4 * 70000 | Sus scrofa |
300000 | - |
gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
complete recovery of enzyme denatured with 8 M urea on dialysis or 50fold dilution. The enzyme loses its secondary structure at urea concentrations of 2 M and higher, whereas the tertiary structure is minimally perturbed below 4 M urea. Dialysis of the enzyme denatured with 1 M guanidine-HCl results in 15-20% recovery of enzyme activity. In 1 M guanidine HCl the enzyme loses both its secondary and tertiary structures and dissociates into monomers of 70000 Da. Both monomeric and dimeric species are observed after 24 h dialysis of the enzyme denatured with guanidine-HCl. Both the monomeric and dimeric forms recovered after dialysis are active | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-Ala-p-nitroanilide + H2O | - |
Sus scrofa | N-acetyl-Ala + p-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | both monomeric and dimeric species are observed after 24 h dialysis of the enzyme denatured with guanidine-HCl. Both the monomeric and dimeric forms recovered after dialysis are active | Sus scrofa |
tetramer | 4 * 70000 | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
APH | - |
Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
461 | - |
N-acetyl-Ala-p-nitroanilide | pH 7.5 | Sus scrofa |