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Literature summary for 3.4.17.B1 extracted from

  • Bec, N.; Villa, A.; Tortora, P.; Mozhaev, V.V.; Balny, C.; Lange, R.
    Enhanced stability of carboxypeptidase from Sulfolobus solfataricus at high pressure (1996), Biotechnol. Lett., 18, 483-488.
No PubMed abstract available
Search for more literature for 3.4.17.B1

General Stability

General Stability Organism
after dilution of the carboxypeptidase in MES buffer, pH 6.5, in the absence of glycerol and 2-mercaptoethanol, the enzyme undergoes a slow loss of activity Saccharolobus solfataricus
in the absence of glycerol and beta-mercaptoethanol, at 50°C, the enzyme undergoes a slow thermal inactivation upon dilution in an aqueous buffer at pH 6.5. This loss of activity can be inhibited when the enzyme is maintained at high pressure. At higher temperatures, higher pressures (up to 400 MPa) are required to maintain the enzyme in its active state. Saccharolobus solfataricus
inactivation is strongly temperature and pressure dependent Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys pH 6.5, 50°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P80092
-
-
Saccharolobus solfataricus P2 P80092
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
-20°C, buffer containing 10 mM imidazole-HCl, 10 mM potassium acetate and 23 mM Tris-HCl, pH 7.2, in the presence of 2 mM beta-mercaptoethanol and 50% (v/v) glycerol Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys + H2O
-
 Saccharolobus solfataricus N-[3-(2-Furylacryloyl)]-L-Ala + L-Lys
-
 ?
N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys + H2O
-
 Saccharolobus solfataricus P2 N-[3-(2-Furylacryloyl)]-L-Ala + L-Lys
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 stable for several days at room temperature in buffer containing 10 mM imidazole-HCl, 10 mM potassium acetate and 23 mM Tris-HCl, pH 7.2, in the presence of 2 mM 2-mercaptoethanol and 50% (v/v) glycerol Saccharolobus solfataricus
50
-
 in the absence of glycerol and 2-mercaptoethanol, at 50°C, the enzyme undergoes a slow thermal inactivation upon dilution in an aqueous buffer at pH 6.5. This loss of activity can be inhibited when the enzyme is maintained at high pressure. At higher temperatures, higher pressures (up to 400 MPa) are required to maintain the enzyme in its active state Saccharolobus solfataricus
50
-
 after 1 h of incubation at 50°C and 1 MPa in the absence of substrate, the activity is decreased by 30%. This effect is abolished when the enzyme is incubated at 200 MPa. Activity loss at atmospheric pressure is about 7% in 10 min, the activity can be stabilized completely at 300 MPa Saccharolobus solfataricus
90
-
 enzyme looses activity at the rate of 25% in 10 min, a pressure raise of 400 MPa results in a 4fold decrease of the inactivation rate Saccharolobus solfataricus