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Literature summary for 3.4.17.B1 extracted from

  • Fusi, P.; Villa, M.; Burlini, N.; Tortora, P.; Guerritore, A.
    Intracellular proteases from the extremely thermophilic archaebacterium Sulfolobus solfataricus (1991), Experientia, 47, 1057-1060.
No PubMed abstract available
Search for more literature for 3.4.17.B1

Activating Compound

Activating Compound Comment Organism Structure
leupeptin activates Saccharolobus solfataricus
additional information not affected by EDTA, pepstatin, and bestatin, enzyme activity is increased 3 to 4fold when yeast extract instead of glucose is used in the cell culture medium Saccharolobus solfataricus
phosphoramidon activates Saccharolobus solfataricus
PMSF activates Saccharolobus solfataricus
tosyl-lysine chloromethyl ketone activates Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
additional information not affected by EDTA, pepstatin, and bestatin Saccharolobus solfataricus
o-phenanthroline
-
 Saccharolobus solfataricus
p-(chloromercuri)benzene sulfonate
-
 Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptide + H2O Saccharolobus solfataricus
-
 ?
-
 ?
peptide + H2O Saccharolobus solfataricus MT-4 / DSM 5833
-
 ?
-
 ?
protein + H2O Saccharolobus solfataricus
-
 peptides
-
 ?
protein + H2O Saccharolobus solfataricus MT-4 / DSM 5833
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.022
-
 crude cell extract, cells grown on glucose, exponential phase Saccharolobus solfataricus
0.029
-
 crude cell extract, cells grown on glucose, stationary phase Saccharolobus solfataricus
0.079
-
 crude cell extract, cells grown on yeast extract, stationary phase Saccharolobus solfataricus
0.095
-
 crude cell extract, cells grown on yeast extract, exponential phase Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Asp + H2O
-
 Saccharolobus solfataricus ?
-
 ?
benzyloxycarbonyl-Asp + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
 ?
peptide + H2O
-
 Saccharolobus solfataricus ?
-
 ?
peptide + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
 ?
protein + H2O
-
 Saccharolobus solfataricus peptides
-
 ?
protein + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 peptides
-
 ?

Synonyms

Synonyms Comment Organism
M20.008 Merops-ID Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 stable at, 15 min, pH 7.0 Saccharolobus solfataricus