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Literature summary for 3.4.17.8 extracted from
- PBP5 complementation of a PBP3 deficiency in Enterococcus hirae (2006), J. Bacteriol., 188, 6298-6307.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pbp5, complementation of a PBP5-deficient Enterococcus hirae strain SL1 and a PBP3-deficient Enterococcus hirae strain SL2 | Enterococcus faecium |
| gene pbp5, expression of wild-type and mutant enzymes in Escherichia coli, complementation of the pbp5-deficient mutant strain SL1 and of the pbp3-deficient strain SL2 by wild-type and mutant enzymes, recombinant strains, overview | Enterococcus hirae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K172N/R173N | site-directed mutagenesis, M1 mutant, the mutant enzyme shows altered complementation of the pbp3-deficient mutant strain SL2 compared to the wild-type enzyme, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1, overview | Enterococcus hirae |
| K199Q | site-directed mutagenesis, M5 mutant, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1 | Enterococcus hirae |
| K199Q/K203Q | site-directed mutagenesis, M2 mutant, the mutant enzyme shows altered complementation of the pbp3-deficient mutant strain SL2 compared to the wild-type enzyme, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1, overview | Enterococcus hirae |
| K203Q | site-directed mutagenesis, M3 mutant, the mutant enzyme shows altered complementation of the pbp3-deficient mutant strain SL2 compared to the wild-type enzyme, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1, overview | Enterococcus hirae |
| R173N | site-directed mutagenesis, M4 mutant, the mutant enzyme shows complementation of the pbp5-deficient mutant strain SL1 | Enterococcus hirae |
| S422A | site-directed mutagenesis, M6 mutant, inactive mutant | Enterococcus hirae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecium | - |
gene pbp5 | - |
| Enterococcus hirae | - |
wild-type strain ATCC 9790, gene pbp5 | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PBP5 | - |
Enterococcus faecium |
| PBP5 | - |
Enterococcus hirae |
| penicillin-binding protein 5 | - |
Enterococcus faecium |
| penicillin-binding protein 5 | - |
Enterococcus hirae |
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