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Literature summary for 3.4.17.8 extracted from

  • Diaz-Maurino, T.; Nieto, M.; Perkins, H.R.
    Membrane-bound DD-carboxypeptidases from Bacillus megaterium KM (1974), Biochem. J., 134, 391-402.
No PubMed abstract available
Search for more literature for 3.4.17.8

Inhibitors

Inhibitors Comment Organism Structure
acetyl-Gly3-D-Ala-D-Asp
-
 Priestia megaterium
carbenicillin
-
 Priestia megaterium
Cu2+
-
 Priestia megaterium
Hg2+
-
 Priestia megaterium
Methicillin
-
 Priestia megaterium
penicillin G
-
 Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
 Priestia megaterium
0.4
-
 UDP-N-acetylmuramoyl-L-Ala-gamma-D-Glu-L-mesodiaminopimelyl-D-Ala-D-Ala
-
 Priestia megaterium

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Priestia megaterium
Co2+ activation Priestia megaterium
Mg2+ activation Priestia megaterium
Mn2+ activation Priestia megaterium
Zn2+ activation Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine + H2O Priestia megaterium
-
 ?
-
 ?
UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine + H2O Priestia megaterium KM / ATCC 13632
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
 KM
-
Priestia megaterium KM / ATCC 13632
-
 KM
-

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Priestia megaterium
-

Storage Stability

Storage Stability Organism
-20°C, membrane preparation, pH 7.8 or 5.0, 1 year Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information influence of residue preceeding C-terminal D-Ala-D-Ala on rate of hydrolysis Priestia megaterium ?
-
 ?
additional information influence of residue preceeding C-terminal D-Ala-D-Ala on rate of hydrolysis Priestia megaterium KM / ATCC 13632 ?
-
 ?
Nalpha, Nepsilon-diacetyl L-Lys-D-Ala-Gly + H2O
-
 Priestia megaterium Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + Gly
-
 ?
Nalpha, Nepsilon-diacetyl L-Lys-D-Ala-Gly + H2O
-
 Priestia megaterium KM / ATCC 13632 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + Gly
-
 ?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Priestia megaterium Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
 ?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Priestia megaterium KM / ATCC 13632 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
 ?
UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine + H2O
-
 Priestia megaterium ?
-
 ?
UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine + H2O
-
 Priestia megaterium KM / ATCC 13632 ?
-
 ?
UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-L-mesodiaminopimelyl-D-Ala-D-Ala + H2O
-
 Priestia megaterium UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-L-mesodiaminopimelyl-D-Ala + D-Ala
-
 ?
UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-L-mesodiaminopimelyl-D-Ala-D-Ala + H2O
-
 Priestia megaterium KM / ATCC 13632 UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-L-mesodiaminopimelyl-D-Ala + D-Ala
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 up to Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Priestia megaterium