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Literature summary for 3.4.17.3 extracted from

  • Levin, Y.; Skidgel, R.A.; Erdös, E.G.
    Isolation and characterization of the subunits of human plasma carboxypeptidase N (kininase i) (1982), Proc. Natl. Acad. Sci. USA, 79, 4618-4622.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.17.3

General Stability

General Stability Organism
plasmin and trypsin cleave the enzyme to lower molecular weight fragments Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 hippuryl-Arg
-
 Homo sapiens
0.25
-
 Benzoyl-Ala-Lys
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000 + 2 * 83000, the 48000 Da subunit is as active as the intact enzyme, the 83000 Da subunit is inactive but stabilizes the 48000 Da subunit, SDS-PAGE Homo sapiens
83000
-
 2 * 48000 + 2 * 83000, the 48000 Da subunit is as active as the intact enzyme, the 83000 Da subunit is inactive but stabilizes the 48000 Da subunit, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification the 83000 Da subunit contains carbohydrate Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
-
 Homo sapiens Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe + Arg
-
 ?
benzoyl-Ala-Lys + H2O
-
 Homo sapiens ?
-
 ?
Hippuryl-L-Arg + H2O
-
 Homo sapiens Hippuric acid + L-Arg
-
 ?
kallikrein + H2O plasma or urinary Homo sapiens ?
-
 ?
plasmin + H2O
-
 Homo sapiens ?
-
 ?
trypsin + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 48000 + 2 * 83000, the 48000 Da subunit is as active as the intact enzyme, the 83000 Da subunit is inactive but stabilizes the 48000 Da subunit, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 room temperature, pH 4-5, 1 h, the 48000 Da subunit loses 94% of its activity, the intact enzyme loses 9% of its activity Homo sapiens
37
-
 2 h, stable Homo sapiens