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Literature summary for 3.4.17.24 extracted from

  • Wu, H.Y.; Rong, Y.; Correia, K.; Min, J.; Morgan, J.I.
    Comparison of the enzymatic and functional properties of three cytosolic carboxypeptidase family members (2015), J. Biol. Chem., 290, 1222-1232 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.17.24

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.074
-
 biotin-Glu-Glu-Glu-Gly-Glu-Glu pH 7.4, 37°C Mus musculus
0.076
-
 biotin-Glu-Glu-Gly-Glu-Glu-Glu pH 7.4, 37°C Mus musculus
0.15
-
 biotin-Glu-Glu pH 7.4, 37°C Mus musculus
0.18
-
 biotin-Glu-Glu-Glu pH 7.4, 37°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-

Organism

Organism UniProt Comment Textmining
Mus musculus Q641K1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biotin-Glu-Glu + H2O Nna1 can metabolized substrates with two or more glutamate residues. It is more active against longer chain substrates Mus musculus ?
-
 ?
biotin-Glu-Glu-Glu + H2O Nna1 can metabolized substrates with two or more glutamate residues. It is more active against longer chain substrates Mus musculus ?
-
 ?
biotin-Glu-Glu-Glu-Glu + H2O Nna1 can metabolized substrates with two or more glutamate residues. It is more active against longer chain substrates Mus musculus ?
-
 ?
biotin-Glu-Glu-Glu-Glu-Glu + H2O Nna1 can metabolized substrates with two or more glutamate residues. It is more active against longer chain substrates Mus musculus ?
-
 ?
biotin-Glu-Glu-Glu-Gly-Glu-Glu + H2O Nna1 can metabolized substrates with two or more glutamate residues. It is more active against longer chain substrates Mus musculus ?
-
 ?
biotin-Glu-Glu-Gly-Glu-Glu-Glu + H2O Nna1 can metabolized substrates with two or more glutamate residues. It is more active against longer chain substrates Mus musculus ?
-
 ?
tubulin-polyglutamate + H2O the enzyme (Nna1) metabolizes the polyglutamate side chain of tubulin. It removes the C-terminal glutamate from substrates with two or more glutamates. The enzyme exhibits a monoglutamase activity when aspartic acid precedes a single glutamate Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
CCP1
-
 Mus musculus
Nna1
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
36.9
-
 biotin-Glu-Glu pH 7.4, 37°C Mus musculus
94.7
-
 biotin-Glu-Glu-Glu-Gly-Glu-Glu pH 7.4, 37°C Mus musculus
195.1
-
 biotin-Glu-Glu-Gly-Glu-Glu-Glu pH 7.4, 37°C Mus musculus
257.6
-
 biotin-Glu-Glu-Glu pH 7.4, 37°C Mus musculus

General Information

General Information Comment Organism
physiological function the C-terminal regions of tubulins undergo multiple forms of post-translational modifications, which, in addition to polyglutamylation, include removal and addition of a C-terminal tyrosine residue, addition and trimming of polyglycine side chains, and proteolytic processing to yield DELTA2 tubulin. The enzyme (Nna1) is involved in several of these processes, as it not only removes polyglutamate chains from tubulin, but also cleaves synthetic substrates that mimic the detyrosinated and DELTA2 forms of the C terminus of alpha-tubulin the enzyme is involved in the posttranslational process of polyglutamylation, where they catalyze the removal of polyglutamate side chains Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information the enzyme (Nna1) exhibits higher kcat/Km when substrates contain nearby acidic amino acids Mus musculus
250
-
 biotin-Glu-Glu pH 7.4, 37°C Mus musculus
1280
-
 biotin-Glu-Glu-Glu-Gly-Glu-Glu pH 7.4, 37°C Mus musculus
1430
-
 biotin-Glu-Glu-Glu pH 7.4, 37°C Mus musculus
1570
-
 biotin-Glu-Glu-Gly-Glu-Glu-Glu pH 7.4, 37°C Mus musculus