Cloned (Comment) | Organism |
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expression in Sf9 cell | Mus musculus |
Organism | UniProt | Comment | Textmining |
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Mus musculus | Q09M02 | - |
- |
Reaction | Comment | Organism | Reaction ID |
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This is a subfamily of enzymes that cleave C-terminal and/or side chain amino acids from tubulins. The dual-specificity enzymes can cleave both alpha- and gamma-linked L-glutamate from tubulins, removing the posttranslationally added polyglutamyl side chains from the C-terminal regions. In addition, the enzyme removes two glutamate residues from the C-terminus of beta-tubulin and detyrosinated alpha-tubulin (from which the C-terminal L-tyrosine has been removed by EC 3.4.17.17, tubulinyl-Tyr carboxypeptidase). The latter is cleaved to delta2-tubulin and further to delta3-tubulin. | enzyme additionally cleaves alpha-linked Glu from the C-terminus of alpha-tubulin and alpha-linked Glu residues from the side chain of alpha- and beta-tubulin | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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beta2b-tubulin + H2O | - |
Mus musculus | ? | incubation with isoform CCP5 causes decreases of the peaks corresponding to multi- and monoglutamylated beta2b-tubulin and a dramatic increase of the peak corresponding to beta2b-tubulin without Glu on the side chain | ? | |
DATAEEEGEMYE(gamma-(E)2)-DDDEESEAQGPK + 2 H2O | synthetic beta3-tubulin-based 24-residue C-terminal peptide with two side chain Glu residues | Mus musculus | DATAEEEGEMYEDDDEESEAQGPK + 2 L-Glu | about 30% removal of Glu side chains | ? | |
DATAEEEGEMYE(gamma-(E)3)-DDDEESEAQGPK + 3 H2O | synthetic beta3-tubulin-based 24-residue C-terminal peptide with three side chain Glu residues | Mus musculus | DATAEEEGEMYEDDDEESEAQGPK + 3 L-Glu | - |
? | |
DATAEEEGEMYE(gamma-(E)4)-DDDEESEAQGPK + 4 H2O | synthetic beta3-tubulin-based 24-residue C-terminal peptide with four side chain Glu residues | Mus musculus | DATAEEEGEMYEDDDEESEAQGPK + 4 L-Glu | - |
? | |
DATAEEEGEMYE(gamma-E)-DDDEESEAQGPK + H2O | synthetic beta3-tubulin-based 24-residue C-terminal peptide with one side chain Glu residue | Mus musculus | DATAEEEGEMYEDDDEESEAQGPK + L-Glu | complete removal of Glu side chain | ? | |
DELTA2-tubulin + H2O | i.e. tubulin lacking two C-terminal amino acids | Mus musculus | DELTA3-tubulin + L-glutamate | i.e. tubulin lacking two C-terminal amino acids | ? | |
detyrosinated alpha-tubulin + H2O | - |
Mus musculus | DELTA2-tubulin + L-glutamate | treatment with isoform CCP5 leads to a decrease in the peak intensities of the multi- as well as monoglutamylated alpha-tubulin forms and an increase in the peak intensity of the form lacking all Glu residues on the side chain | ? | |
additional information | isoform CCP5 removes multiple glutamate residues and the branch point glutamate from the side chains of porcine brain alpha- and beta-tubulin. CCP5 excises C-terminal glutamates from detyrosinated alpha-tubulin. The enzyme also removes multiple glutamate residues from side chains and C-termini of paclitaxel-stabilized microtubules. CCP5 both shortens and removes side chain glutamates from synthetic peptides corresponding to the C-terminal region of beta3-tubulin | Mus musculus | ? | - |
? | |
polymerized microtubules + H2O | polymerized microtubules prepared from MDA-MB-231 cells grown on a coverslip. Isoform CCP5 eliminates GT335 signals and diminishes poly(E) signals on polymerized tubulin | Mus musculus | ? | - |
? | |
tubulin + H2O | porcine brain tubulin. Isoform CCP5 can process paclitaxel-treated tubulin, and the activity of CCP5 in the presence of paclitaxel is comparable with the activity in the absence of the drug | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
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CCP5 | - |
Mus musculus |
deglutamylase | - |
Mus musculus |