Cloned (Comment) | Organism |
---|---|
expression of extracellular domain and wild-type, both as FLAG-tagged proteins, in Spodotera frugiperda Sf9 cells via baculovirus infection | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ac-GDYSHCSPLRYYPWWKCTYPDPEGGG-NH2 | strong inhibition, most potent inhibitory peptide, i.e. DX600 | Homo sapiens | |
additional information | construction of 6 constrained peptide libraries, selected from peptide libraries displayed on phage, peptides, 21-27 amino acids, with inhibitory effects on the enzyme, specificity and stability, selection of inhibitory sequence motifs, best CXPXRXXPWXXC, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin I + H2O | Homo sapiens | - |
angiotensin-(1-9) + Leu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BYF1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and extracellular domain as FLAG-tagged proteins from Sf9 cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)acetyl-YVADAPK(2,4-dinitrophenyl)-OH + H2O | synthetic fluorogenic caspase-1 substrate | Homo sapiens | (7-methoxycoumarin-4-yl)acetyl-YVADAP + N6-(2,4-dinitrophenyl)-L-lysine | - |
? | |
angiotensin I + H2O | - |
Homo sapiens | angiotensin-(1-9) + Leu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACE2 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki values of peptides from constrained peptide libraries | Homo sapiens | |
0.0028 | - |
Ac-GDYSHCSPLRYYPWWKCTYPDPEGGG-NH2 | pH 8.0, room temperature with substrate angiotensin I, pH 7.4, room temperature with substrate (7-methoxycoumarin-4-yl)acetyl-YVADAPK(2,4-dinitrophenyl)-OH | Homo sapiens |