Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.17.23 extracted from

  • Vickers, C.; Hales, P.; Kaushik, V.; Dick, L.; Gavin, J.; Tang, J.; Godbout, K.; Parsons, T.; Baronas, E.; Hsieh, F.; Acton, S.; Patane, M.; Nichols, A.; Tummino, P.
    Hydrolysis of biological peptides by human angiotensin-converting enzyme-related carboxypeptidase (2002), J. Biol. Chem., 277, 14838-14843.
    View publication on PubMed
Search for more literature for 3.4.17.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Spodoptera frugiperda Sf9 cells via infection with baculovirus Homo sapiens

General Stability

General Stability Organism
Zn2+ stabilizes Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.147
-
 (7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH pH 6.5, room temperature Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- enhances activity by about 10fold Homo sapiens
F- enhances activity by about 10fold Homo sapiens
additional information no effect of Br- Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89600
-
 recombinant enzyme, MALDI-TOF mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
angiotensin I + H2O Homo sapiens
-
 angiotensin-(1-9) + Leu
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BYF1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Sf9 cells, to near homogeneity Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
angiotensin II + H2O = angiotensin-(1-7) + L-phenylalanine ACE2 catalytic efficiency is 400-fold higher with angiotensin II (1–8) as a substrate than with angiotensin I (1–10). ACE2 also efficiently hydrolyzes des-Arg9-bradykinin, but it does not hydrolyze bradykinin Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH + H2O synthetic fluorogenic substrate Homo sapiens (7-methoxycoumarin-4-yl)acetyl-AP + N6-(2,4-dinitrophenyl)-L-lysine
-
 ?
(7-methoxycoumarin-4-yl)acetyl-YVADAPK(2,4-dinitrophenyl)-OH + H2O synthetic fluorogenic caspase-1 substrate Homo sapiens (7-methoxycoumarin-4-yl)acetyl-YVADAP + N6-(2,4-dinitrophenyl)-L-lysine
-
 ?
angiotensin I + H2O
-
 Homo sapiens angiotensin-(1-9) + Leu
-
 ?
angiotensin II + H2O 400fold higher activity than with angiotensin I Homo sapiens angiotensin-(1-7) + Phe
-
 ?
apelin-13 + H2O
-
 Homo sapiens apelin-12 + Phe
-
 ?
apelin-36 + H2O
-
 Homo sapiens apelin-35 + Phe
-
 ?
dynorphin A 1-13 + H2O
-
 Homo sapiens dynorphin A 1-12 + Lys
-
 ir
ghrelin + H2O
-
 Homo sapiens ghrelin minus C-terminal amino acid + arginine
-
 ir
KRPPGSPF + H2O i.e. Lys-des-Arg-bradykinin Homo sapiens KRPPGSP + Phe
-
 ir
neocasomorphin + H2O
-
 Homo sapiens neocasomorphin minus C-terminal amino acid + isoleucine
-
 ir
neurotensin-(1-8) + H2O
-
 Homo sapiens neurotensin-(1-7) + arginine
-
 ir
RPPGSPF + H2O i.e. des-Arg-bradykinin Homo sapiens RPPGSP + Phe i.e. des-Arg-bradykinin-(1-7) ir
YPVEPFI + H2O i.e. beta-casomorphin Homo sapiens YPVEPF + Ile
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 room temperature, assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 recombinant enzyme, at room temperature, stable for 6 h Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 angiotensin I pH 6.5, room temperature Homo sapiens
6840
-
 (7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH pH 6.5, room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Homo sapiens