Cloned (Comment) | Organism |
---|---|
expression in Spodoptera frugiperda Sf9 cells via infection with baculovirus | Homo sapiens |
General Stability | Organism |
---|---|
Zn2+ stabilizes | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.147 | - |
(7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH | pH 6.5, room temperature | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cl- | enhances activity by about 10fold | Homo sapiens | |
F- | enhances activity by about 10fold | Homo sapiens | |
additional information | no effect of Br- | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89600 | - |
recombinant enzyme, MALDI-TOF mass spectrometry | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin I + H2O | Homo sapiens | - |
angiotensin-(1-9) + Leu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BYF1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Sf9 cells, to near homogeneity | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
angiotensin II + H2O = angiotensin-(1-7) + L-phenylalanine | ACE2 catalytic efficiency is 400-fold higher with angiotensin II (18) as a substrate than with angiotensin I (110). ACE2 also efficiently hydrolyzes des-Arg9-bradykinin, but it does not hydrolyze bradykinin | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH + H2O | synthetic fluorogenic substrate | Homo sapiens | (7-methoxycoumarin-4-yl)acetyl-AP + N6-(2,4-dinitrophenyl)-L-lysine | - |
? | |
(7-methoxycoumarin-4-yl)acetyl-YVADAPK(2,4-dinitrophenyl)-OH + H2O | synthetic fluorogenic caspase-1 substrate | Homo sapiens | (7-methoxycoumarin-4-yl)acetyl-YVADAP + N6-(2,4-dinitrophenyl)-L-lysine | - |
? | |
angiotensin I + H2O | - |
Homo sapiens | angiotensin-(1-9) + Leu | - |
? | |
angiotensin II + H2O | 400fold higher activity than with angiotensin I | Homo sapiens | angiotensin-(1-7) + Phe | - |
? | |
apelin-13 + H2O | - |
Homo sapiens | apelin-12 + Phe | - |
? | |
apelin-36 + H2O | - |
Homo sapiens | apelin-35 + Phe | - |
? | |
dynorphin A 1-13 + H2O | - |
Homo sapiens | dynorphin A 1-12 + Lys | - |
ir | |
ghrelin + H2O | - |
Homo sapiens | ghrelin minus C-terminal amino acid + arginine | - |
ir | |
KRPPGSPF + H2O | i.e. Lys-des-Arg-bradykinin | Homo sapiens | KRPPGSP + Phe | - |
ir | |
neocasomorphin + H2O | - |
Homo sapiens | neocasomorphin minus C-terminal amino acid + isoleucine | - |
ir | |
neurotensin-(1-8) + H2O | - |
Homo sapiens | neurotensin-(1-7) + arginine | - |
ir | |
RPPGSPF + H2O | i.e. des-Arg-bradykinin | Homo sapiens | RPPGSP + Phe | i.e. des-Arg-bradykinin-(1-7) | ir | |
YPVEPFI + H2O | i.e. beta-casomorphin | Homo sapiens | YPVEPF + Ile | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
room temperature, assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
recombinant enzyme, at room temperature, stable for 6 h | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
angiotensin I | pH 6.5, room temperature | Homo sapiens | |
6840 | - |
(7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH | pH 6.5, room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Homo sapiens |