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Literature summary for 3.4.17.23 extracted from

  • Yan, Z.H.; Ren, K.J.; Wang, Y.; Chen, S.; Brock, T.A.; Rege, A.A.
    Development of intramolecularly quenched fluorescent peptides as substrates of angiotensin-converting enzyme 2 (2003), Anal. Biochem., 312, 141-147.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Sf21 cells via infection with baculovirus, mIgG-tagged protein Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- required, highest activity at 1.5 M NaCl Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
angiotensin I + H2O Homo sapiens
-
angiotensin-(1-9) + Leu
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BYF1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Sf21 cells as mIgG-tagged protein Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O
-
Homo sapiens angiotensin-(1-9) + Leu
-
?
RPPGSPF + H2O i.e. des-Arg-bradykinin Homo sapiens RPPGSP + Phe i.e. des-Arg-bradykinin-(1-7) ir
TBC5046 + H2O synthetic fluorogenic peptide, i.e. des-Arg-bradykinin with N-terminal o-aminobenzoic acid and a 3-nitrophenylalanine instead of Phe at the C-terminus Homo sapiens o-aminobenzoic acid-des-Arg-bradykinin-(1-7) + 3-nitrophenylalanine
-
ir

Synonyms

Synonyms Comment Organism
ACE2
-
Homo sapiens
angiotensin-converting enzyme-related carboxypeptidase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 activity drops sharply at pH 8.0, substantial activity at pH 4.5-6.5, inactive at pH 9.0 Homo sapiens