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Literature summary for 3.4.17.21 extracted from

  • Klusak, V.; Barinka, C.; Plechanovova, A.; Mlcochova, P.; Konvalinka, J.; Rulisek, L.; Lubkowski, J.
    Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography, and computational methods (2009), Biochemistry, 48, 4126-4138.
    View publication on PubMed
Search for more literature for 3.4.17.21

Application

Application Comment Organism
medicine glutamate carboxypeptidase II is a therapeutic target for neurodegeneration and prostate cancer Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Drosophila Schneiders S2 cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of GCPII(E424A) in complex with N-acetyl-L-aspartyl-L-glutamate is determined at 1.70 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
E424A mutant, complete loss of catalytic activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-L-aspartyl-L-glutamate + H2O Homo sapiens
-
 N-acetyl-L-aspartate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q04609
-
-

Purification (Commentary)

Purification (Comment) Organism
purification consists of four steps, QAE-Sephadex A50 batch chromatography, chromatography on a Source 15S column, affinity chromatography using lentil lectin Sepharose, and gel filtration on a Superdex 200 column Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
prostate
-
 Homo sapiens
-
small intestine
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-L-aspartyl-L-glutamate + H2O
-
 Homo sapiens N-acetyl-L-aspartate + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
GCPII
-
 Homo sapiens
glutamate carboxypeptidase II
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity assay Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 N-acetyl-L-aspartyl-L-glutamate
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 activity assay Homo sapiens