Cloned (Comment) | Organism |
---|---|
stable expression of wild-type and mutant enzymes in S2 cells, secretion of the enzyme, which is prevented by blocking the N-glycosylation | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N121A | site-directed mutagenesis of an N-glycosylation site, nearly inactive enzyme | Homo sapiens |
N140A | site-directed mutagenesis of an N-glycosylation site, nearly inactive enzyme | Homo sapiens |
N153A | site-directed mutagenesis of an N-glycosylation site, nearly inactive enzyme | Homo sapiens |
N195A | site-directed mutagenesis of an N-glycosylation site, nearly inactive enzyme | Homo sapiens |
N336A | site-directed mutagenesis of an N-glycosylation site leads to 45% reduced activity compared to the wild-type enzyme | Homo sapiens |
N476A | site-directed mutagenesis of an N-glycosylation site leads to 70% reduced activity compared to the wild-type enzyme | Homo sapiens |
N51A | site-directed mutagenesis of an N-glycosylation site leads to 75% reduced activity compared to the wild-type enzyme | Homo sapiens |
N638A | site-directed mutagenesis of an N-glycosylation site, nearly inactive enzyme | Homo sapiens |
N76A | site-directed mutagenesis of an N-glycosylation site leads to 25% reduced activity compared to the wild-type enzyme | Homo sapiens |
T640A | site-directed mutagenesis of an N-glycosylation site, nearly inactive enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000066 | - |
N-acetyl-alpha-L-aspartyl-L-glutamate | recombinant mutant N195A | Homo sapiens | |
0.0001 | - |
N-acetyl-alpha-L-aspartyl-L-glutamate | recombinant mutant N121A | Homo sapiens | |
0.000321 | - |
N-acetyl-alpha-L-aspartyl-L-glutamate | recombinant wild-type enzyme | Homo sapiens | |
0.000334 | - |
N-acetyl-alpha-L-aspartyl-L-glutamate | recombinant mutant N638A | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | type II membrane protein | Homo sapiens | 16020 | - |
synaptic cleft | - |
Homo sapiens | 43083 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-alpha-L-aspartyl-L-glutamate + H2O | Homo sapiens | the enzyme is responsible for cleavage of NAAG to yield free glutamate | N-acetyl-alpha-L-aspartate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | identification of the N-glycosylation sites, e.g. N121, N153, N195, N638, and N140, the enzyme is heavily glycosylated | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates | T640 is involved in catalysis, the N-glycosylation status is important for actalytic activity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
central nervous system | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
peripheral nervous system | - |
Homo sapiens | - |
prostate gland | prostate form of the enzyme is termed prostate-specific membrane antigen | Homo sapiens | - |
small intestine | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-alpha-L-aspartyl-L-glutamate + H2O | the enzyme is responsible for cleavage of NAAG to yield free glutamate | Homo sapiens | N-acetyl-alpha-L-aspartate + L-glutamate | - |
? | |
N-acetyl-alpha-L-aspartyl-L-glutamate + H2O | i.e. NAAG | Homo sapiens | N-acetyl-alpha-L-aspartate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GCPII | - |
Homo sapiens |
N-Acetylated alpha-linked acidic dipeptidase | - |
Homo sapiens |
NAAG-hydrolyzing activity | - |
Homo sapiens |
NAALADase | - |
Homo sapiens |
Prostate-specific membrane antigen | - |
Homo sapiens |
PSMA | - |
Homo sapiens |