Literature summary for 3.4.17.20 extracted from

Marx, P.F.; Plug, T.; Havik, S.R.; Moergelin, M.; Meijers, J.C.
The activation peptide of thrombin-activatable fibrinolysis inhibitor: a role in activity and stability of the enzyme? (2009), J. Thromb. Haemost., 7, 445-452.

Search for more literature for 3.4.17.20

Application

Application	Comment	Organism
molecular biology	results demonstrate that the activation peptide is not required for TAFIa activity or for stabilization of the enzyme, but solely for stabilization of the zymogen	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
R302Q	mutant aggregates similarly to wild-type protein	Homo sapiens
T325I/T329I/H333Y/H335Q	inactive mutant has a 70fold increased half-life and a 3fold to 5fold increased antifibrinolytic function as compared to wild-type, mutant aggregates into large, insoluble complexes that can be removed by centrifugation	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	proteolytic enzymes activate TAFI into TAFIa, by cleaving off the N-terminal activation peptide (amino acids 1-92), from the enzyme moiety. TAFIa is active both before and after removal of the activation peptide, and the half-life of TAFIa is identical in the two preparations	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
using a Nik-9H10-Sepharose column, coupled with the antibody Nig9H10 whose epitope is located on the activation peptide of TAFI	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hippuryl-L-arginine + H2O	-	Homo sapiens	hippuric acid + L-arginine	-	?

Synonyms

Synonyms	Comment	Organism
TAFIa	-	Homo sapiens
thrombin-activatable fibrinolysis inhibitor	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)