Literature summary for 3.4.17.20 extracted from

Stroemqvist, M.; Hansson, L.; Andersson, J.O.; Johansson, T.; Edlund, M.; Enoksson, M.; Goossens, F.; Scharpe, S.; Hendriks, D.
Properties of recombinant human plasma procarboxypeptidase U produced in mammalian and insect cells (2004), Clin. Chim. Acta, 347, 49-59.

Search for more literature for 3.4.17.20

Cloned(Commentary)

Cloned (Comment)	Organism
expression of pro-CPU in Spodoptera frugiperda Sf21 and Trichoplusia ni H5 insect cells, the recombinant enzymes show different glycosylation patterns, expression rates, overview	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	immobilization of purified recombinnat Pro-CPU on Sepharose for specific antibody purification	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
potato carboxypeptidase inhibitor	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	plasma	Homo sapiens	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the enzyme is a metallopeptidase	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	x * 35000, processed mature glycosylated enzyme, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q96IY4	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Homo sapiens
proteolytic modification	the enzyme is synthesized as zymogen, activation by thrombin and thrombomodulin at pH 8.5	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
native pro-CPU from human plasma by two steps of ion exchange chromatography, plasminogen affinity chromatography, gel filtration, and anion exchange chromatography, recombinant pro-CPU from Sf21 and H5 insect cells using the same method as for the native enzyme, to homogeneity	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
blood plasma	-	Homo sapiens	-

Subunits

Subunits	Comment	Organism
?	x * 35000, processed mature glycosylated enzyme, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
plasma procarboxypeptidase U	-	Homo sapiens
proCPU	-	Homo sapiens
TAFIa	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)