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Literature summary for 3.4.17.2 extracted from

  • Arolas, J.L.; Popowicz, G.M.; Lorenzo, J.; Sommerhoff, C.P.; Huber, R.; Aviles, F.X.; Holak, T.A.
    The three-dimensional structures of tick carboxypeptidase inhibitor in complex with A/B carboxypeptidases reveal a novel double-headed binding mode (2005), J. Mol. Biol., 350, 489-498.
    View publication on PubMed
Search for more literature for 3.4.17.2

Application

Application Comment Organism
drug development the enzyme is a target for design of active site inhibitors based on the structure of tick carboxypeptidase inhibitor, a potential antithrombolytic drug Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the zymogen in Pichia pastoris straim KM71 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant enzyme in complex with tick carboxypeptidase inhibitor, sitting drop vapour diffusion method, mixing of equal volumes of protein and reservoir solutions, the latter containing 0.1 M bis-Tris. pH 5.5, 0.2 M lithium sulfate monohydrate, and 25% w/v PEG 3350, a few weeks at 20°C, X-ray diffraction structure determination and analysis at 2.0 A resolution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
tick carboxypeptidase inhibitor TCI, from Rhipicephalus bursa, recombinantly expressd in Escherichia coli strain BL21(DE3) periplasm and purification by cation exchange and adsorption chromatography, binding structure at the active site, overview Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P15086
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification cleavage and activation of the recombinant zymogen by trypsin Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
B carboxypeptidase
-
 Homo sapiens
CPB
-
 Homo sapiens